Document Detail

Fish oil enhances the antiproliferative effect of 1alpha,25-dihydroxyvitamin D3 on liver cancer cells.
MedLine Citation:
PMID:  19667153     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Laboratory and epidemiological studies have indicated that 1alpha,25-dihydroxyvitamin D3 [1alpha,25(OH)2D3] and dietary omega 3 (omega3)-polyunsaturated fatty acids (PUFAs) are capable of inhibiting the proliferation of various cancer cells. MATERIALS AND METHODS: Human hepatoblastoma cells (HepG2) were treated with 1alpha,25(OH)2D3 and fish oil alone and in combination. Cell proliferation was measured either by the uptake of [3H]-thymidine into DNA or by counting the cell numbers using a hemocytometer. RESULTS: The HepG2 cell proliferation was inhibited by 1alpha,25(OH)2D3 and fish oil in a dose-dependent manner. The lowest effective concentration of 1alpha,25(OH)2D3 was 10(-7) M and 10(-8) M using the [3H]-thymidine incorporation method and the cell counting method, respectively. Fish oil also caused a significant inhibition in HepG2 cell proliferation at 25 microg/mL. When HepG2 cells were treated with 1alpha,25(OH)2D3 in combination with fish oil, it was found that fish oil increased the antiproliferative effect of 1alpha,25(OH)2D3 on HepG2 cell growth compared to treatment with 1alpha,25(OH)2D3 alone. CONCLUSION: 1alpha,25(OH)2D3 could be used to treat hepatocellular carcinoma (HCC). However, the major side-effect of hypercalcemia limits its use. An enhanced 1alpha,25(OH)2D3-induced inhibition of HepG2 cell proliferation in the presence of PUFAs in the form of fish oil suggests that a lower concentration of 1alpha,25(OH)2D3 could be used to treat hepatocellular carcinoma in the presence of PUFAs to decrease the risk of hypercalcemia caused by high concentrations of 1alpha,25(OH)2D3.
Kun-Chun Chiang; Kelly S Persons; Nawfal W Istfan; Michael F Holick; Tai C Chen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  29     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-11     Completed Date:  2009-09-01     Revised Date:  2010-04-16    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  3591-6     Citation Subset:  IM    
Department of Surgery, Chang Gung Memorial Hospital;Chang Gung University, Taiwan, R.O.C.
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MeSH Terms
Calcitriol / pharmacology*
Cell Line, Tumor
Cell Proliferation / drug effects*
Drug Synergism
Fish Oils / pharmacology*
Liver Neoplasms / pathology*
Reg. No./Substance:
0/Fish Oils; 32222-06-3/Calcitriol
Erratum In:
Anticancer Res.2010 Jan;30(1):277
Anticancer Res.2010 Mar;30(3):1033

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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