Document Detail

Fish oil and antioxidants alter the composition and function of circulating mononuclear cells in Crohn disease.
MedLine Citation:
PMID:  15531659     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Crohn disease (CD) is associated with osteoporosis and other extraintestinal manifestations that might be mediated by cytokines from circulating (peripheral blood) mononuclear cells (PBMCs). Fish oil rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) reduces disease activity in patients with CD with raised laboratory markers of inflammation and in healthy subjects alters PBMC function. OBJECTIVE: We investigated the effect of fish oil plus antioxidants on cytokine production by PBMCs from patients with CD with raised C-reactive protein concentrations (>/=6.9 mg/L) or erythrocyte sedimentation rates (>/=18 mm/h). DESIGN: A randomized placebo-controlled trial of fish oil (2.7 g EPA and DHA/d; n = 31) or placebo (olive oil; n = 31) for 24 wk was conducted in patients with CD. The fish-oil group additionally received an antioxidant preparation (vitamins A, C, and E and selenium). Exclusion criteria included corticosteroid use. Fatty acid composition was measured by gas chromatography. Production of tumor necrosis factor alpha, interferon gamma (IFN-gamma), and prostaglandin E(2) (PGE(2)) was measured by enzyme-linked immunosorbent assays after stimulation with mitogen and endotoxin (lipopolysaccharide). RESULTS: Fish-oil plus antioxidant dietary supplementation was associated with higher EPA and DHA incorporation into PBMCs (P < 0.001) and lower arachidonic acid (P = 0.006) and lower production of IFN-gamma by mitogen-stimulated PBMCs (P = 0.012) and of PGE(2) by lipopolysaccharide-stimulated PBMCs (P = 0.047). CONCLUSION: Dietary supplementation with fish oil plus antioxidants is associated with modified PBMC composition and lower production of PGE(2) and IFN-gamma by circulating monocytes or macrophages. The response of extraintestinal manifestations of CD should be investigated in a randomized controlled trial.
Timothy M Trebble; Nigel K Arden; Stephen A Wootton; Philip C Calder; Mark A Mullee; David R Fine; Mike A Stroud
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Publication Detail:
Type:  Clinical Trial; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of clinical nutrition     Volume:  80     ISSN:  0002-9165     ISO Abbreviation:  Am. J. Clin. Nutr.     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-11-08     Completed Date:  2004-12-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376027     Medline TA:  Am J Clin Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1137-44     Citation Subset:  AIM; IM    
Institute of Human Nutrition, School of Medicine, University of Southampton, Southampton, United Kingdom.
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MeSH Terms
Antioxidants / administration & dosage,  therapeutic use*
Crohn Disease / drug therapy*,  metabolism
Dietary Fats / therapeutic use*
Double-Blind Method
Fish Oils / administration & dosage,  therapeutic use*
Leukocytes, Mononuclear / drug effects*,  metabolism
Middle Aged
Prostaglandins / biosynthesis*
Reg. No./Substance:
0/Antioxidants; 0/Dietary Fats; 0/Fish Oils; 0/Prostaglandins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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