Document Detail


Fish oil alters T-lymphocyte proliferation and macrophage responses in Walker 256 tumor-bearing rats.
MedLine Citation:
PMID:  16472986     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: We investigated the effect of the dietary ratio of omega-6 to omega-3 polyunsaturated fatty acids (PUFAs) from postweaning until adulthood on T-lymphocyte proliferation, T-lymphocyte subpopulations (helper and cytotoxic), and production of cytotoxic mediators by macrophages in tumor-bearing rodents. METHODS: Weanling male Wistar rats received a normal low-fat (40 g/kg of diet) chow diet or a high-fat (300 g /kg) diet that included fish or sunflower oil or blends of fish and sunflower oils to yield omega-6:omega-3 PUFA ratios of approximately 6:1, 30:1, and 60:1 ad libitum. After 8 wk, 50% of rats in each group were inoculated with 1 mL of 2 x 10(7) Walker 256 cells. Fourteen days after tumor inoculation, animals were killed and lymphocytes and macrophages were obtained for study. RESULTS: The diets richest in omega-6 PUFA resulted in higher proliferation of thymus, spleen, and gut-associated lymphocytes compared with the chow diet irrespective of tumor burden. In contrast, the fish oil diet resulted in lower proliferation of thymus and spleen lymphocytes compared with the chow diet. Diets rich in omega-6 PUFA decreased the proportion of CD8+ lymphocytes. In non-tumor-bearing and tumor-bearing rats, hydrogen peroxide production by macrophages was highest in rats that consumed diets high in omega-3 PUFAs. Superoxide and nitric oxide production were little affected by the dietary ratio of omega-6 to omega-3 PUFAs. CONCLUSION: Dietary omega-6 and omega-3 PUFA contents alter immune function in non-tumor-bearing and tumor-bearing rats. The omega-3 PUFAs decreased T-cell proliferation but increased hydrogen peroxide production compared with omega-6 PUFAs. Decreased tumor growth and cachexia and increased survival previously reported for fish oil in Walker 256 tumor-bearing rats may be related to improved macrophage function rather than to improved T-cell function.
Authors:
Nathalia Pizato; Sandro Bonatto; Maressa Piconcelli; Lauro M de Souza; Guilherme L Sassaki; Katya Naliwaiko; Everson A Nunes; Rui Curi; Philip C Calder; Luiz Claudio Fernandes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-10
Journal Detail:
Title:  Nutrition (Burbank, Los Angeles County, Calif.)     Volume:  22     ISSN:  0899-9007     ISO Abbreviation:  Nutrition     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-13     Completed Date:  2006-08-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8802712     Medline TA:  Nutrition     Country:  United States    
Other Details:
Languages:  eng     Pagination:  425-32     Citation Subset:  IM    
Affiliation:
Department of Physiology, Federal University of Paraná, Paraná, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carcinoma 256, Walker / immunology,  metabolism*
Dose-Response Relationship, Drug
Fatty Acids, Omega-3 / administration & dosage,  pharmacology*
Fatty Acids, Omega-6 / administration & dosage,  pharmacology*
Fish Oils* / administration & dosage,  pharmacology
Hydrogen Peroxide / metabolism
Lymphocyte Activation
Macrophages / drug effects,  immunology*
Male
Plant Oils / administration & dosage,  pharmacology
Random Allocation
Rats
Rats, Wistar
T-Lymphocytes / drug effects,  immunology*
Chemical
Reg. No./Substance:
0/Fatty Acids, Omega-3; 0/Fatty Acids, Omega-6; 0/Fish Oils; 0/Plant Oils; 7722-84-1/Hydrogen Peroxide; 8001-21-6/sunflower seed oil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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