Document Detail

Fish fast skeletal muscle tropomyosins show species-specific thermal stability.
MedLine Citation:
PMID:  15967697     Owner:  NLM     Status:  MEDLINE    
Tropomyosin (TM) was isolated from the fast skeletal muscle of six fish species, whose amino acid sequences of this protein have already been revealed. The thermal stability of these TMs was measured by differential scanning calorimetry (DSC) and circular dichroism (CD), while the molecular weights were measured by mass spectrometry. The results showed clear differences in thermostability among these fish TMs, though the identity of amino acid sequences was more than 93.3%. Therefore, only a few amino acid substitutions could affect the overall stability of the TM molecule. Especially, several residues located on the molecular surface were considered to be responsible for such stability difference. In contrast, the molecular weights of these TMs as measured by mass spectrometry were higher than those calculated from amino acid composition, suggesting the presence of post-translational modification(s) which could also affect their thermal stability.
Ming-Chih Huang; Yoshihiro Ochiai
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  141     ISSN:  1096-4959     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-07-19     Completed Date:  2005-10-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  461-71     Citation Subset:  IM    
Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan.
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MeSH Terms
Amino Acid Sequence
Calorimetry, Differential Scanning
Circular Dichroism
Molecular Sequence Data
Muscle, Skeletal / chemistry*
Protein Denaturation / genetics,  physiology
Sequence Alignment
Sequence Homology, Amino Acid
Species Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tropomyosin / chemistry*,  genetics,  isolation & purification
Reg. No./Substance:

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