| Fish Oil-Supplemented Parenteral Nutrition Prolongs Survival While Beneficially Altering Phospholipids Fatty Acids Composition and Modulating Immune Function in Rat Sepsis. | |
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MedLine Citation:
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PMID: 21522042 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We investigated the therapeutic effects of parenteral fish oil on survival, fatty acids profile in plasma and erythrocytes membranes, T lymphocyte subsets, and plasma cytokines in a rat model of sepsis. Adult male Sprague-Dawley rats were subjected to cecal ligation and puncture-induced sepsis. For recovery, central venous catheterization was performed 2 days before sepsis induced. Rats were randomly assigned to receive normal saline (n=20), or total parenteral nutrition containing a standard soybean oil emulsion (n=20), or fish oil-supplemented total parenteral nutrition (n=20) at the onset of sepsis for 5 days. In control group, rats were challenged by sham operation and underwent appropriate control treatment (n=10). Sepsis led to a high mortality and body weight lost compared with sham operation. Total parenteral nutrition supplemented with fish oil, but not without fish oil, improved the survival compared with normal saline. Furthermore, parenteral infusion of fish oil increased the concentrations of eicosapentaenoic acid and docosahexaenoic acid, as well as the ratio of (eicosapentaenoic acid + docosahexaenoic acid) to arachidonic acid both in plasma and erythrocyte membrane. Additionally, fish oil-supplemented total parenteral nutrition improved the percentages of CD3+ and CD3+CD4+ T cells, as well as the CD4+/CD8+ ratio in spleen. Meanwhile, the percentage of regulatory T cells (CD4+CD25+Foxp3+) among CD4+ T cells was reduced by fish oil-supplemented total parenteral nutrition. Fish oil-supplemented total parenteral nutrition attenuated the production of high-mobility group box 1 and interleukin-10 in plasma. Moreover, parenteral fish oil decreased the bacterial loads in peritoneal lavage, blood, lung, and spleen. The present study suggests fish oil-supplemented total parenteral nutrition initiated at the onset of sepsis improves survival, beneficially alters the lipids profile in plasma and erythrocyte membrane, modulates immune function and regulates inflammatory response in a rat model. |
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Authors:
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Shougen Cao; Jianan Ren; Liqun Sun; Guosheng Gu; Yujie Yuan; Jieshou Li |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-8 |
Journal Detail:
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Title: Shock (Augusta, Ga.) Volume: - ISSN: 1540-0514 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-4-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9421564 Medline TA: Shock Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, 210002, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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