Document Detail


Fish and mammalian phagocytes differentially regulate pro-inflammatory and homeostatic responses in vivo.
MedLine Citation:
PMID:  23110059     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phagocytosis is a cellular mechanism that is important to the early induction of antimicrobial responses and the regulation of adaptive immunity. At an inflammatory site, phagocytes serve as central regulators for both pro-inflammatory and homeostatic anti-inflammatory processes. However, it remains unclear if this is a recent evolutionary development or whether the capacity to balance between these two seemingly contradictory processes is a feature already displayed in lower vertebrates. In this study, we used murine (C57BL/6) and teleost fish (C. auratus) in vitro and in vivo models to assess the evolutionary conservation of this dichotomy at a site of inflammation. At the level of the macrophage, we found that teleost fish already displayed divergent pro-inflammatory and homeostatic responses following internalization of zymosan or apoptotic bodies, respectively, and that these were consistent with those of mice. However, fish and mice displayed significant differences in vivo with regards to the level of responsiveness to zymosan and apoptotic bodies, the identity of infiltrating leukocytes, their rate of infiltration, and the kinetics and strength of resulting antimicrobial responses. Unlike macrophages, significant differences were identified between teleost and murine neutrophilic responses. We report for the first time that activated murine, but not teleost neutrophils, possess the capacity to internalize apoptotic bodies. This internalization translates into reduction of neutrophil ROS production. This may play an important part in the recently identified anti-inflammatory activity that mammalian neutrophils display during the resolution phase of inflammation. Our observations are consistent with continued honing of inflammatory control mechanisms from fish to mammals, and provide added insights into the evolutionary path that has resulted in the integrated, multilayered responses that are characteristic of higher vertebrates.
Authors:
Aja M Rieger; Jeffrey D Konowalchuk; Leon Grayfer; Barbara A Katzenback; Jeffrey J Havixbeck; Moira D Kiemele; Miodrag Belosevic; Daniel R Barreda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-23
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-10-30     Completed Date:  2013-04-17     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e47070     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Female
Goldfish
Inflammation / immunology*,  metabolism
Mice
Mice, Inbred C57BL
Neutrophils / immunology,  metabolism
Phagocytes / metabolism,  microbiology*
Phagocytosis / physiology
Reactive Oxygen Species / metabolism
Zymosan / metabolism
Chemical
Reg. No./Substance:
0/Reactive Oxygen Species; 9010-72-4/Zymosan
Comments/Corrections

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