| Fisetin prevents fluoride- and dexamethasone-induced oxidative damage in osteoblast and hippocampal cells. | |
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MedLine Citation:
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PMID: 22198064 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Fluoride intoxication and dexamethasone treatment produce deleterious effects in bone and brain. The aim of this study was to evaluate the effect of fluoride (F) and dexamethasone (Dex) co-exposure on oxidative stress and apoptosis in osteoblast-like MC3T3-E1 and hippocampal HT22 cell lines. Co-exposure to F and Dex resulted in a concentration-dependent decrease in cell viability, induction of apoptosis and increased generation of reactive oxygen species (ROS) and nitric oxide (NO) following 72h of incubation. Fluoride-induced apoptosis in MC3T3-E1 and HT22 cells was attenuated by catalase and L-NNMA, indicating a role for H(2)O(2) and NO as mediators of cytotoxicity. Dexamethasone-induced apoptosis was associated with H(2)O(2) generation in both cell lines and it was attenuated during co-incubation with catalase. These data indicate that co-exposure to F and Dex amplifies their respective cytotoxicity in H(2)O(2)- and NO-dependent manner. As flavonoid fisetin prevented F- and Dex-induced cytotoxicity the potential role of this product in pharmacology and diet may be considered. |
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Authors:
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Iwona Inkielewicz-Stepniak; Marek W Radomski; Michal Wozniak |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-17 |
Journal Detail:
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Title: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association Volume: - ISSN: 1873-6351 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8207483 Medline TA: Food Chem Toxicol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Ltd. |
Affiliation:
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Department of Medical Chemistry, Medical University of Gdansk, Poland. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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