Document Detail


First proficiency testing to evaluate the ability of European Union National Reference Laboratories to detect staphylococcal enterotoxins in milk products.
MedLine Citation:
PMID:  12733489     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The European Commission has designed a network of European Union-National Reference Laboratories (EU-NRLs), coordinated by a Community Reference Laboratory (CRL), for control of hygiene of milk and milk products (Council Directive 92/46/ECC). As a common contaminant of milk and milk products such as cheese, staphylococcal enterotoxins are often involved in human outbreaks and should be monitored regularly. The main tasks of the EU-CRLs were to select and transfer to the EU-NRLs a reference method for detection of enterotoxins, and to set up proficiency testing to evaluate the competency of the European laboratory network. The first interlaboratory exercise was performed on samples of freeze-dried cheese inoculated with 2 levels of staphylococcal enterotoxins (0.1 and 0.25 ng/g) and on an uninoculated control. These levels were chosen considering the EU regulation for staphylococcal enterotoxins in milk and milk products and the limit of detection of the enzyme-linked immunosorbent assay test recommended in the reference method. The trial was conducted according to the recommendations of ISO Guide 43. Results produced by laboratories were compiled and compared through statistical analysis. Except for data from 2 laboratories for the uninoculated control and cheese inoculated at 0.1 ng/g, all laboratories produced satisfactory results, showing the ability of the EU-NRL network to monitor the enterotoxin contaminant.
Authors:
Jacques-Antoine Hennekinne; Martine Gohier; Tiphaine Maire; Christiane Lapeyre; Bertrand Lombard; Sylviane Dragacci
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  86     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2003 Mar-Apr
Date Detail:
Created Date:  2003-05-01     Completed Date:  2003-07-14     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  332-9     Citation Subset:  IM    
Affiliation:
Agence Française de Sécurité Sanitaire des Aliments, Laboratoire d'Etudes, Microbial Toxins Unit, 10 Rue Pierre Curie, 94704 Maisons-Alfort Cedex, France. ja.hennekinne@afssa.fr
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Analysis of Variance
Cheese / microbiology
Dairy Products / microbiology*
Electrophoresis, Polyacrylamide Gel
Enterotoxins / analysis*
European Union
Food Microbiology*
Reference Standards
Staphylococcus / chemistry*
Chemical
Reg. No./Substance:
0/Enterotoxins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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