| First live offspring born in superovulated sika deer (Cervus nippon) after embryo vitrification. | |
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MedLine Citation:
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PMID: 22980091 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The rapid growth in sika deer (Cervus nippon) farming and interest in their conservation is an impetus for development of embryo transfer (ET) procedures. However, a paucity of research has prevented widespread application of ET in this species. The objective of the present study was to establish a multiple ovulation and ET procedure with both fresh and vitrified embryos in sika deer. Multiparous weaned hinds (N = 18) were used as embryo donors during the reproductive season of 2008 at a local breeding farm in China. Estrus was synchronized in donors and recipients (N = 38) by inserting a controlled internal drug release for 12 days (insertion = Day 0). Superovulation was induced with a total of 320 mg of NIH-FSH-P1 (Folltropin-V; Bioniche, Belleville, ON, Canada) given as 40 mg im every 12 h from the afternoon of Day 9 to the morning of Day 13. After estrus was detected, donors were artificially inseminated using a transcervical technique. The embryo recovery rate was 76.8% (63/82), including 1.6% (1/63), 77.8% (49/63), and 1.6% (1/63) blastocysts, morula, and eight-cell embryos, respectively. After transfer of fresh and vitrified embryos, pregnancy rates were 85.7% and 61.6% and birth rates were 64.3% and 53.9% (P > 0.05). In conclusion, we developed a satisfactory multiple ovulation and ET procedure in farmed sika deer using vitrified embryos. |
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Authors:
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L Wang; G B Zhou; W Q Shi; J M Shi; X Z Tian; C Gao; L Zhang; S E Zhu; T T Zhang; S M Zeng; G S Liu |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Theriogenology Volume: 78 ISSN: 1879-3231 ISO Abbreviation: Theriogenology Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-09-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0421510 Medline TA: Theriogenology Country: United States |
Other Details:
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Languages: eng Pagination: 1627-32 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Elsevier Inc. All rights reserved. |
Affiliation:
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Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China; Animal Husbandry and Veterinary Station of Beijing, Beijing, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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