Document Detail


First cell cycles of sea urchin Paracentrotus lividus are dramatically impaired by exposure to extremely low-frequency electromagnetic field.
MedLine Citation:
PMID:  16957026     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Exposure of fertilized eggs of the sea urchin Paracentrotus lividus to an electromagnetic field of 75-Hz frequency and low amplitudes (from 0.75 to 2.20 mT of magnetic component) leads to a dramatic loss of synchronization of the first cell cycle, with formation of anomalous embryos linked to irregular separation of chromatids during the mitotic events. Because acetylcholinesterase (ACHE) is thought to regulate the embryonic first developmental events of the sea urchin, its enzymatic activity was assayed in embryo homogenates and decreased by 48% when the homogenates were exposed to the same pulsed field. This enzymatic inactivation had a threshold of about 0.75 +/- 0.01 mT. The same field threshold was found for the effect on the formation of anomalous embryos of P. lividus. Moreover, ACHE inhibitors seem to induce the same teratological effects as those caused by the field, while blockers of acetylcholine (ACh) receptors are able to antagonize those effects. We conclude that one of the main causes of these dramatic effects on the early development of the sea urchin by field exposure could be the accumulation of ACh due to ACHE inactivation. The crucial role of the membrane in determining the conditions for enzyme inactivation is discussed.
Authors:
Silvia Ravera; Carla Falugi; Daniela Calzia; Isidoro M Pepe; Isabella Panfoli; Alessandro Morelli
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Publication Detail:
Type:  Journal Article     Date:  2006-09-06
Journal Detail:
Title:  Biology of reproduction     Volume:  75     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-11-28     Completed Date:  2007-02-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  948-53     Citation Subset:  IM    
Affiliation:
Department of Biology, University of Genoa, 16132 Genova, Italy.
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MeSH Terms
Descriptor/Qualifier:
Acetylcholinesterase / metabolism
Animals
Cell Cycle / radiation effects
Dose-Response Relationship, Radiation
Electromagnetic Fields / adverse effects*
Embryo, Nonmammalian / cytology,  enzymology,  radiation effects
Fluorescent Dyes
Indoles
Paracentrotus
Zygote / cytology*,  enzymology,  radiation effects*
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Indoles; 47165-04-8/DAPI; EC 3.1.1.7/Acetylcholinesterase

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