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First case series of emerging Rickettsial neonatal sepsis identified by polymerase chain reaction-based deoxyribonucleic acid sequencing.
MedLine Citation:
PMID:  24064639     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Purpose: To detect and identify the aetiological agent in the peripheral blood from the cases of neonatal sepsis. Materials and Methods: Four neonates from geographically different regions of South India presented with signs of neonatal sepsis and all the routine clinical and laboratory investigations were performed. Blood culture by Bac T Alert 3D was negative. To establish the aetiology, polymerase chain reaction (PCR) for eubacterial genome and subsequent amplification with Gram positive and Gram negative primers were performed followed by deoxyribonucleic acid (DNA) sequencing. Results: PCR for the detection of eubacterial genome was positive in all the four neonates and further amplification with designed Gram positive and Gram negative primers revealed the presence of Gram negative bacteria. The amplicons were identified as Orientia tsutsugamushi in three neonates and Coxiella burnetti in the other neonate. Multalin analysis was done to further characterise the strain variation among the three strains. Conclusion: PCR-based DNA sequencing is a rapid and reliable diagnostic tool to identify the aetiological agents of neonatal sepsis. This is the first case series of emerging Rickettsial neonatal sepsis in India .
Authors:
P Aarthi; R Bagyalakshmi; K R Mohan; M Krishna; M Nitin; H N Madhavan; S Kalyani
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Indian journal of medical microbiology     Volume:  31     ISSN:  1998-3646     ISO Abbreviation:  Indian J Med Microbiol     Publication Date:    2013 Oct-Dec
Date Detail:
Created Date:  2013-09-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8700903     Medline TA:  Indian J Med Microbiol     Country:  India    
Other Details:
Languages:  eng     Pagination:  343-8     Citation Subset:  IM    
Affiliation:
Larsen and Toubro Microbiology Research Centre, Kamal Nayan Bajaj Research Centre, Vision Research Foundation, Nungambakkam, Chennai, Tamil Nadu, India.
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