Document Detail

First Phylogenetic Analysis Of Bluetongue Virus Serotype 4 Field Isolates From Argentina.
MedLine Citation:
PMID:  23152367     Owner:  NLM     Status:  Publisher    
Bluetongue (BT) is an insect-transmitted viral disease of ruminant species and represents a major barrier for international trade of animals and their products. Bluetongue virus (BTV) has a genome composed by ten linear segments of double stranded RNA (dsRNA) which codify for at least ten different viral proteins. In South America, serological evidence for the presence of BTV has been found in Peru, Argentina, Brazil, Ecuador and Chile. Brazil and Argentina are the only countries where BTV has been isolated. In Brazil, a unique BTV isolate from serotype 12 has been reported and in Argentina five BTV 4 isolates has been obtained from cattle without clinical signs. Three of them were isolated during the years 1999-2001 and two new isolates were obtained as part of the present work. This study describes sequence comparisons and phylogenetic analyses of Seg-2, 3, 6, 7 and 10 from the first Argentinean field isolates of BTV. The analysis of segments 2 and 6 resulted in a single cluster of Argentinean sequences into the serotype 4 clade. In addition, the Argentinean sequences grouped within the nucleotype A clade, along with reference strains. The analysis of segments 3, 7 and 10 showed that the Argentinean isolates grouped into western topotype, indicating that the circulating virus had an African/European origin. The phylogenetic analysis revealed that Argentinean sequences presented a Southamerican genetic identity, assessed in every segment analysis, suggesting an independent lineage evolution.
Danilo Legisa; Fernanda Gonzalez; Gabriel de Stefano; Ariel Pereda; Maria J Dus Santos
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-14
Journal Detail:
Title:  The Journal of general virology     Volume:  -     ISSN:  1465-2099     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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