| Finger-stick Blood Sampling Methodology for the Determination of Exercise-induced Lymphocyte Apoptosis. | |
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MedLine Citation:
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PMID: 21403625 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Exercise is a physiological stimulus capable of inducing apoptosis in immune cells. To date, various limitations have been identified with the measurement of this phenomenon, particularly relating to the amount of time required to isolate and treat a blood sample prior to the assessment of cell death. Because of this, it is difficult to determine whether reported increases in immune cell apoptosis can be contributed to the actual effect of exercise on the system, or are a reflection of the time and processing necessary to eventually obtain this measurement. In this article we demonstrate a rapid and minimally invasive procedure for the analysis of exercise-induced lymphocyte apoptosis. Unlike other techniques, whole blood is added to an antibody panel immediately upon obtaining a sample. Following the incubation period, red blood cells are lysed and samples are ready to be analyzed. The use of a finger-stick sampling procedure reduces the volume of blood required, and minimizes the discomfort to subjects. |
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Authors:
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James Navalta; Brian McFarlin; Richard Simpson; Elizabeth Fedor; Holly Kell; Scott Lyons; Scott Arnett; Mark Schafer |
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Publication Detail:
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Type: Journal Article Date: 2011-02-24 |
Journal Detail:
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Title: Journal of visualized experiments : JoVE Volume: - ISSN: 1940-087X ISO Abbreviation: J Vis Exp Publication Date: 2011 |
Date Detail:
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Created Date: 2011-03-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101313252 Medline TA: J Vis Exp Country: United States |
Other Details:
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Languages: eng Pagination: - Citation Subset: IM |
Affiliation:
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Department of Kinesiology, Recreation, and Sport, Western Kentucky University. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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