Document Detail


Fine-scale genetic structure among genetic individuals of the clone-forming monotypic genus Echinosophora koreensis (Fabaceae).
MedLine Citation:
PMID:  16675603     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND AIMS: For rare endemics or endangered plant species that reproduce both sexually and vegetatively it is critical to understand the extent of clonality because assessment of clonal extent and distribution has important ecological and evolutionary consequences with conservation implications. A survey was undertaken to understand clonal effects on fine-scale genetic structure (FSGS) in two populations (one from a disturbed and the other from an undisturbed locality) of Echinosophora koreensis, an endangered small shrub belonging to a monotypic genus in central Korea that reproduces both sexually and vegetatively via rhizomes.
METHODS: Using inter-simple sequence repeats (ISSRs) as genetic markers, the spatial distribution of individuals was evaluated using Ripley's L(d)-statistics and quantified the spatial scale of clonal spread and spatial distribution of ISSR genotypes using spatial autocorrelation analysis techniques (join-count statistics and kinship coefficient, F(ij)) for total samples and samples excluding clones.
KEY RESULTS: A high degree of differentiation between populations was observed (phi(ST(g)) = 0.184, P < 0.001). Ripley's L(d)-statistics revealed a near random distribution of individuals in a disturbed population, whereas significant aggregation of individuals was found in an undisturbed site. The join-count statistics revealed that most clones significantly aggregate at < or = 6-m interplant distance. The Sp statistic reflecting patterns of correlograms revealed a strong pattern of FSGS for all four data sets (Sp = 0.072-0.154), but these patterns were not significantly different from each other. At small interplant distances (< or = 2 m), however, jackknifed 95% CIs revealed that the total samples exhibited significantly higher F(ij) values than the same samples excluding clones.
CONCLUSION: The strong FSGS from genets is consistent with two biological and ecological traits of E. koreensis: bee-pollination and limited seed dispersal. Furthermore, potential clone mates over repeated generations would contribute to the observed high F(ij) values among genets at short distance. To ensure long-term ex situ genetic variability of the endangered E. koreensis, individuals located at distances of 10-12 m should be collected across entire populations of E. koreensis.
Authors:
Jae Min Chung; Byeung Cheun Lee; Jin Seok Kim; Chong-Wook Park; Mi Yoon Chung; Myong Gi Chung
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Publication Detail:
Type:  Journal Article     Date:  2006-05-04
Journal Detail:
Title:  Annals of botany     Volume:  98     ISSN:  0305-7364     ISO Abbreviation:  Ann. Bot.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-06-19     Completed Date:  2006-08-22     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0372347     Medline TA:  Ann Bot     Country:  England    
Other Details:
Languages:  eng     Pagination:  165-73     Citation Subset:  IM    
Affiliation:
Division of Specimen and Genetic Resources, National Arboretum, Korea Forest Service, Gyeonggi Province, 487-821, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Conservation of Natural Resources
Data Interpretation, Statistical
Fabaceae / genetics*,  physiology
Genetic Markers
Genetic Variation*
Genotype
Korea
Repetitive Sequences, Nucleic Acid
Reproduction, Asexual
Chemical
Reg. No./Substance:
0/Genetic Markers
Comments/Corrections

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