| Fine mapping of Leishmania major susceptibility Locus lmr2 and evidence of a role for Fli1 in disease and wound healing. | |
| | |
MedLine Citation:
|
PMID: 20368343 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Genetic linkage studies of the host response to Leishmania major, the causative agent of cutaneous leishmaniasis, have identified significant genetic complexity in humans and mice. In the mouse model, multiple loci have been implicated in susceptibility to infection, but to date, the genes underlying these loci have not been identified. We now describe the contribution of a novel candidate gene, Fli1, to both L. major resistance and enhanced wound healing. We have previously mapped the L. major response locus, lmr2, to proximal chromosome 9 in a genetic cross between the resistant C57BL/6 strain and the susceptible BALB/c strain. We now show that the presence of the resistant C57BL/6 lmr2 allele in susceptible BALB/c mice confers an enhanced L. major resistance and wound healing phenotype. Fine mapping of the lmr2 locus permitted the localization of the lmr2 quantitative trait locus to a 5-Mb interval comprising 21 genes, of which microarray analysis was able to identify differential expression in 1 gene-Fli1. Analysis of Fli1 expression in wounded and L. major-infected skin and naïve and infected lymph nodes validated the importance of Fli1 in lesion resolution and wound healing and identified 3 polymorphisms in the Fli1 promoter, among which a GA repeat element may be the important contributor. |
| | |
Authors:
|
Anuratha Sakthianandeswaren; Joan M Curtis; Colleen Elso; Beena Kumar; Tracey M Baldwin; Sash Lopaticki; Lukasz Kedzierski; Gordon K Smyth; Simon J Foote; Emanuela Handman |
Related Documents
:
|
11955643 - Mutations associated with genotypic resistance to antiretroviral therapy in treatment n... 17576693 - Dna bar coding and pyrosequencing to identify rare hiv drug resistance mutations. 18230963 - A novel frameshift mutation (+a) at codon 18 of the beta-globin gene associated with hi... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-04-05 |
Journal Detail:
|
Title: Infection and immunity Volume: 78 ISSN: 1098-5522 ISO Abbreviation: Infect. Immun. Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-05-20 Completed Date: 2010-06-01 Revised Date: 2011-03-03 |
Medline Journal Info:
|
Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: United States |
Other Details:
|
Languages: eng Pagination: 2734-44 Citation Subset: IM |
Affiliation:
|
Ludwig Institute for Cancer Research, PO Box 2008, Royal Melbourne Hospital, Parkville, Victoria 3050, Australia. Anu.Sakthianandeswaren@ludwig.edu.au |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Chromosome Mapping Crosses, Genetic Female Gene Expression Profiling Genetic Loci Genetic Predisposition to Disease* Leishmania major / immunology* Leishmaniasis, Cutaneous / immunology* Mice Mice, Inbred BALB C Mice, Inbred C57BL Polymorphism, Genetic Promoter Regions, Genetic Proto-Oncogene Protein c-fli-1 / physiology* Wound Healing* |
| Chemical | |
Reg. No./Substance:
|
0/Fli1 protein, mouse; 0/Proto-Oncogene Protein c-fli-1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Coupled amino acid deamidase-transport systems essential for Helicobacter pylori colonization.
Next Document: Comparative study of the ability of Leishmania mexicana promastigotes and amastigotes to alter macro...