| Fine distribution of CLOCK protein in hepatocytes of hibernating dormice. | |
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MedLine Citation:
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PMID: 14514414 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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CLOCK protein is a member of the bHLH-PAS family of transcription factors, it is expressed in several tissues including the liver and is essential for normal circadian rhythms. In this study we investigate the distribution of CLOCK protein in hepatocytes of euthermic and hibernating edible dormice Glis glis as well as in hepatocytes taken from the hibernating animals submitted in vitro to experimental conditions mimicking the arousal process. Our results demonstrate that CLOCK protein is expressed in all animals and is mostly located in the nucleus, in particular, on perichromatin fibrils and nucleoli. During deep hibernation CLOCK protein becomes more abundant but an intracellular redistribution occurs: the protein significantly decreases in all cellular compartments, but it accumulates in the amorphous bodies. These nuclear bodies, typical of the hibernating state, probably represent storage sites for CLOCK protein to be quickly used upon arousal. Accordingly, in hepatocytes submitted to in vitro conditions mimicking arousal CLOCK protein levels rapidly reach the euthermic values, while amorphous bodies disappear. |
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Authors:
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M Malatesta; B Baldelli; L Rossi; S Serafini; G Gazzanelli |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: European journal of histochemistry : EJH Volume: 47 ISSN: 1121-760X ISO Abbreviation: Eur J Histochem Publication Date: 2003 |
Date Detail:
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Created Date: 2003-09-29 Completed Date: 2004-05-28 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9207930 Medline TA: Eur J Histochem Country: Italy |
Other Details:
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Languages: eng Pagination: 233-40 Citation Subset: IM |
Affiliation:
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Istituto di Istologia ed Analisi di Laboratorio, University of Urbino, via Zeppi s.n., 61029 Urbino, Italy. malatesta@uniurb.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies / immunology CLOCK Proteins Hepatocytes / metabolism*, ultrastructure Hibernation Liver / cytology, metabolism Liver Extracts Male Microscopy, Immunoelectron Rodentia / metabolism* Trans-Activators / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Liver Extracts; 0/Trans-Activators; EC 2.3.1.48/CLOCK Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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