Document Detail


Finding the needles in the haystack: mapping constitutive proteolytic events in vivo.
MedLine Citation:
PMID:  17822382     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Our quest to understand the complex inner workings of the cell depends on the development of new technologies that allow the study of global regulatory events as they happen within their native cellular environment. Post-translational processing of proteins by proteases is one such regulatory process that can control many aspects of basic cell biology. In this issue of the Biochemical Journal, Timmer et al. describe a new proteomic approach that can be used to globally monitor constitutive proteolytic events in vivo. Using bacterial, human, yeast and mouse cells, the authors show that this methodology provides a comprehensive map of constitutive trimming events mediated by regulatory proteases such as methionine aminopeptidase. This study also identifies previously uncharacterized processing events that highlight potential novel regulatory mechanisms mediated by proteolysis.
Authors:
Matthew Bogyo
Publication Detail:
Type:  Comment; Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  407     ISSN:  1470-8728     ISO Abbreviation:  Biochem. J.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-09-07     Completed Date:  2007-11-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  England    
Other Details:
Languages:  eng     Pagination:  e1-2     Citation Subset:  IM    
Affiliation:
Department of Pathology and Department of Microbiology and Immunology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305-5324, USA.
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MeSH Terms
Descriptor/Qualifier:
Aminopeptidases / metabolism
Animals
Humans
Mice
Peptide Hydrolases / metabolism*
Peptide Mapping
Peptides / analysis,  chemistry,  metabolism
Proteome / analysis,  metabolism
Proteomics / methods*
Chemical
Reg. No./Substance:
0/Peptides; 0/Proteome; EC 3.4.-/Peptide Hydrolases; EC 3.4.11.-/Aminopeptidases; EC 3.4.11.18/methionyl aminopeptidase
Comments/Corrections
Comment On:
Biochem J. 2007 Oct 1;407(1):41-8   [PMID:  17650073 ]

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