Document Detail

Filtration of sickle cells: recruitment into a rigid fraction as a function of density and oxygen tension.
MedLine Citation:
PMID:  2732619     Owner:  NLM     Status:  MEDLINE    
The rheologic behavior of sickle cells was evaluated with a constant flow filtration system at 37 degrees C by using filters with pores of 4.8 microns diameter. Analysis of the shape of the pressure-time curves suggested that sickle cells were subdivided into two discrete subpopulations: (1) relatively deformable cells and (2) cells so rigid that they plugged the pores. The analysis allowed calculation of the fraction of cells causing plugging (FP), even when FP represented less than 0.01% of the population. FP rose with falling Po2 level, apparently with the recruitment of progressively less dense cells into the plugging fraction. A density profile for each sample was constructed. When we made the assumption that the plugging cells represented the denser moiety, this profile allowed identification of the threshold Po2 level at which cells of a given density, deoxygenated under these conditions, became unable to traverse the 4.8 microns pore.
E A Schmalzer; R S Manning; S Chien
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of laboratory and clinical medicine     Volume:  113     ISSN:  0022-2143     ISO Abbreviation:  J. Lab. Clin. Med.     Publication Date:  1989 Jun 
Date Detail:
Created Date:  1989-07-14     Completed Date:  1989-07-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375375     Medline TA:  J Lab Clin Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  727-34     Citation Subset:  AIM; IM    
Department of Physiology and Cellular Biophysics, Columbia University College of Physicians and Surgeons, NY.
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MeSH Terms
Anemia, Sickle Cell / blood*
Blood Pressure
Erythrocyte Count
Erythrocyte Deformability*
Erythrocytes / pathology*
Flow Cytometry
Microscopy, Electron, Scanning
Oxygen / blood*
Grant Support
Reg. No./Substance:

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