Document Detail


Filterless fluorometry with enhanced sensitivity.
MedLine Citation:
PMID:  18818990     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe a novel approach for fluorometry with light-emitting-diodes (LEDs) as excitation source that capitalizes on the principle of light shielding and total internal reflection to operate without optical filters. This scheme is practicable, demonstrated to significantly reduce the amount of excitation light overlapping the fluorescence signals at the photodetector, operated best with excitation light applied orthogonal to detection, and permitted higher measurement sensitivity of fluorophore emission.
Authors:
Han Yen Tan; Tuck Wah Ng; Oi Wah Liew
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-09-26
Journal Detail:
Title:  Journal of fluorescence     Volume:  19     ISSN:  1573-4994     ISO Abbreviation:  J Fluoresc     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-19     Completed Date:  2009-05-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9201341     Medline TA:  J Fluoresc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  375-9     Citation Subset:  IM    
Affiliation:
Department of Mechanical Engineering, Monash University, Clayton, VIC, 3800, Australia.
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MeSH Terms
Descriptor/Qualifier:
Fluorescence
Fluorometry / instrumentation*,  methods*
Light

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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