Document Detail


Filterability of subpopulations of leukocytes: effect of pentoxifylline.
MedLine Citation:
PMID:  6743827     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human leukocytes were separated by density into two fractions, one containing predominantly granulocytes (FI) and the other, lymphocytes and monocytes (FII). The filterability of these fractions and their mixture was determined from the pressure measured during constant flow through 5-microns Nuclepore filters. The pressure-time curve of FI indicates the behavior of a relatively homogeneous cell population. The FII pressure-time curve can be analyzed to distinguish the effect of the more numerous and more filterable lymphocytes from that of the sparser but less filterable monocytes. Pressure generated by mixed leukocytes, which had been treated with 1 and 10 mmol/L of pentoxifylline (PTX) or its metabolite I, was substantially less than untreated control; at 10 mmol/L, the pressure was reduced to about 50% of control. PTX appears to affect the filterability of monocytes and polymorphonuclear leukocytes but not lymphocytes. Scanning electron microscopy showed an inhibition of protopod formation in the treated granulocytes. The degree of cell adhesion to the filter, as measured by the number of cells remaining on the outflow side of the filter, was similar in all groups (FI and FII, treated and untreated). The results indicate that the variations in filterability between the leukocyte subpopulations and the improvement by PTX treatment reflect differences in the cells' ability to deform under the test conditions.
Authors:
E A Schmalzer; S Chien
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Blood     Volume:  64     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  1984 Aug 
Date Detail:
Created Date:  1984-08-28     Completed Date:  1984-08-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  542-6     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Separation
Granulocytes / ultrastructure
Humans
Leukocytes / classification*,  drug effects,  ultrastructure
Lymphocytes
Monocytes
Pentoxifylline / pharmacology*
Pressure
Theobromine / analogs & derivatives*
Ultrafiltration*
Grant Support
ID/Acronym/Agency:
HL 07114/HL/NHLBI NIH HHS; HL 16851/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
6493-05-6/Pentoxifylline; 83-67-0/Theobromine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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