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Filming a live cell by scanning electrochemical microscopy: label-free imaging of the dynamic morphology in real time.
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PMID:  22436305     Owner:  NLM     Status:  PubMed-not-MEDLINE    
The morphology of a live cell reflects the organization of the cytoskeleton and the healthy status of the cell. We established a label-free platform for monitoring the changing morphology of live cells in real time based on scanning electrochemical microscopy (SECM). The dynamic morphology of a live human bladder cancer cell (T24) was revealed by time-lapse SECM with dissolved oxygen in the medium solution as the redox mediator. Detailed local movements of cell membrane were presented by time-lapse cross section lines extracted from time-lapse SECM. Vivid dynamic morphology is presented by a movie made of time-lapse SECM images. The morphological change of the T24 cell by non-physiological temperature is in consistence with the morphological feature of early apoptosis. To obtain dynamic cellular morphology with other methods is difficult. The non-invasive nature of SECM combined with high resolution realized filming the movements of live cells.
Michelle Meng-Ni Zhang; Yi-Tao Long; Zhifeng Ding
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Publication Detail:
Type:  Journal Article     Date:  2012-03-21
Journal Detail:
Title:  Chemistry Central journal     Volume:  6     ISSN:  1752-153X     ISO Abbreviation:  Chem Cent J     Publication Date:  2012  
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Created Date:  2012-04-30     Completed Date:  2012-10-02     Revised Date:  2012-11-09    
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Nlm Unique ID:  101314213     Medline TA:  Chem Cent J     Country:  England    
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Languages:  eng     Pagination:  20     Citation Subset:  -    
State Key Laboratory of Bioreactor Engineering and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
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Journal ID (nlm-ta): Chem Cent J
Journal ID (iso-abbrev): Chem Cent J
ISSN: 1752-153X
Publisher: BioMed Central
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Copyright ©2011 Zhang et al
Received Day: 20 Month: 12 Year: 2011
Accepted Day: 21 Month: 3 Year: 2012
collection publication date: Year: 2012
Electronic publication date: Day: 21 Month: 3 Year: 2012
Volume: 6First Page: 20 Last Page: 20
ID: 3338092
Publisher Id: 1752-153X-6-20
PubMed Id: 22436305
DOI: 10.1186/1752-153X-6-20

Filming a live cell by scanning electrochemical microscopy: label-free imaging of the dynamic morphology in real time
Michelle Meng-Ni Zhang12 Email:
Yi-Tao Long1 Email:
Zhifeng Ding2 Email:
1State Key Laboratory of Bioreactor Engineering and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China
2Department of Chemistry, The University of Western Ontario, 1151 Richmond Street, London, ON, N6A 5B7, Canada


The morphology of single live cells reflects the organization of the cytoskeleton and the healthy status of the cell [1]. Monitoring the morphological changes of live cells may provide dynamic information of the cell attachment or cytoskeleton organization [1]. The most commonly used cell imaging method is fluorescent microscopy, which is extremely sensitive and allows the visualization of particular structure or compounds inside the cells [2,3]. On the other hand, the preparation of the specimen is relatively time-consuming, and the live cells are sensitive to photo-damage [3]. As an imaging method with high spatial resolution, atomic force microscopy (AFM) avoids staining process and photo-damage. However, its cantilever tip mechanically damages the soft cells thus is not suitable for time-lapse experiments [4]. As a non-invasive single-cell analysis method, scanning electrochemical microscopy (SECM) has been successfully applied to live cell-imaging due to its high temporal and spatial resolution [5-13]. This technique is based on the measurement of the electrochemical current flowing through the SECM tip, which is usually an ultramicroelectrode (UME). The current detected at the tip is dependent on the separation space between the tip and the cell, therefore morphological information of the live cell can be revealed by the electrochemically mapped images [7-9,11]. Compared to fluorescent microscopy, sample preparation of SECM is simple without any staining or labeling procedure. Unlike AFM, the SECM probe does not need to touch the cell, thus it can carry on time-lapse measurement without mechanically scratching the cell. Nevertheless, most SECM imaging experiments were conducted with the addition of a certain redox mediator, which is usually non- physiologic and undesired [7-9,11,12]. In our previous study [14], we found that dissolved oxygen in the medium solution could be detected by SECM, which provides an opportunity of label-free imaging cellular morphology using dissolved oxygen as the redox mediator. Bladder cancer is the fourth most common cancer of men and the eighth most common cancer of women [15]. Like most of cancers, bladder cancer begins with the mutation of one single cell [16,17]. Investigations of bladder cancer, especially the interaction with anti-cancer drugs, at the single cell level can provide new insight into its physiology, pathology and pharmacology, and promote the development of chemotherapy in response to single-cell behaviours [18]. Herein, the real-time morphological changes of single live T24 cells under non-physiological temperature are revealed by time-lapse SECM with dissolved oxygen as the indicator. While the reactive oxygen species (ROS) released by live cells may interfere with the detection of dissolved oxygen [12,14,19], we determined that under physiological conditions oxygen can be reduced at -0.455 V and hydrogen peroxide can be reduced at -0.745 V, while superoxide is oxidized at +0.055 V. Thus in this research the potential was set at -0.500 V to reduce the dissolved oxygen. We also found that the resting status when the T24 cells do not release ROS can last for up to 5 h [20], which sustains imaging T24 cells with only dissolved oxygen.

The typical SECM setup is illustrated in Figure 1.

The changing morphology of a T24 cell under room temperature was investigated by time-lapse SECM images scanned at about 0.8 μm above the nucleus of the cell with a 5 μm diameter Pt UME biased at -0.500 V vs. Ag/AgCl. (Figure 2b, d and 3) for oxygen reduction [14,21]. The distance between the UME and the cell was calibrated with ferrocenemethanol after the time-lapse SECM experiment [12].

At -0.500 V vs. Ag/AgCl, the dissolved oxygen can be reduced at the diffusion controlled rate at the Pt surface of the UME [14,21]. The time-lapse SECM images in Figure 2 present negative feedback as the current over the T24 cell is lower than the background current, which is the current measured over the Petri dish bottom. This indicates the cell is not releasing anything redox active, and the current flowing through the UME only results from the reduction of dissolved oxygen in medium solution [20]. The T24 cell blocks the dissolved oxygen from diffusing to the Pt surface of the UME (Figure 1), resulting in decreased current over the T24 cell, which is called negative feedback [22,23] in SECM (Figure 2b, d and 3).

In comparison of the SECM images and the 50 × optical micrographs obtained with the same T24 cell (Figure 2), the SECM images present higher spatial resolution than the optical microscopic images. The nucleus (black area) could be clearly distinguished from the membrane (brown area) in the SECM images (Figure 2a and 2b). Conversely, in the first optical microscopic images, the great nuclear area (blue dashed area) and membrane (orange dashed area) can be vaguely distinguished (Figure 2c); 1 h later, only an outline of the cell (blue dashed area) can be seen (Figure 2d). The time-lapse SECM images in Figure 2 demonstrate that the cellular morphology has been changed within 1 h. The membrane has been shrunk and the nucleus has been rounded and compacted (Figure 2b). The morphological change observed in the time-lapse SECM images is in consistence with the time-lapse optical micrographs. It is plausible that in the SECM image in Figure 2a, the current over the black dashed area on the cell membrane is higher than the background current. However, the cross section line drawn from the red dashed position presenting the cellular topography along this position (inset in Figure 2a) shows that this area corresponds to the edge of the cellular membrane, and the current over this area (black triangle pointed current in the cross section line) is no higher than the background current. The color in this area is relatively brighter since the adjacent cell (purple dashed area in Figure 2c) has elevated membrane, and the color scale of a SECM image is adjusted laterally in WiTec software with which the SECM experiments were conducted.

Figure 3 clearly demonstrates the real-time morphological change of the T24 cells over 156 min under room temperature. We could easily distinguish the black condensing nucleus in the center and the significantly shrinking cellular membrane around the nucleus in each image, and observe the dynamic morphological changes of each part. The time-lapse cross section lines drawn from the color dashes in each image of Figure 3 reveal the real-time topographical change of the T24 cell. At the beginning (Figure 3a), the great nuclear area is elevated from the extended cellular membrane; the non-nucleus part of the great nuclear area (green triangles) is higher than the nucleus part (yellow triangles); the whole cell is flat but the surface is uneven and wavy (Figure 3a-d). Then the non-nucleus part of the great nuclear area gradually merges to the nucleus (Figure 3a-f), and cannot be distinguished from the nucleus 30 min later (Figure 3f). The extended cellular membrane slowly shrinks in a worm-like manner, that is, partially elevates and pushes forward into the great nuclear area, for 93 min (red arrows in Figure 3a-k). 93 min later, the cellular topography becomes steady and no obvious change was observed in the next 63 min; the total cell height is raised but the cellular surface is smooth (Figure 3l-p). As can be seen from the lowest current value in each cross section line, the cell height was found to fluctuate periodically in a small range during the 156 min. The real-time morphological changes observed here with time-lapse SECM are consistent with the morphological features of early apoptosis [24], which suggests that the prolonged exposure to room temperature may induce apoptosis of T24 cells. An additional movie file shows the dynamic morphology of the T24 cell in more detail (see Additional file 1).


T24 cells were supplied by American Type Culture Collection (ATCC, Manassas, VA, USA). The T24 cells were cultured in DMEM (Dulbecco's Modified Eagle's Medium) supplemented with 4 mM L-glutamine, 100 units/ml of penicillin, 100 μg/ml of streptomycin, and 10% fetal bovine serum (FBS). All the culture media and supplement were obtained from Gibco (Invitrogen, Burlington, ON, Canada). The cells were incubated at 37°C and 5% CO2 over night before SECM experiments. Cell culture was performed with plastic tissue culture tools (Becton, Dickinson and Company. Mississauga, ON, Canada). The cells were washed with Opti-MEM (no phenol red, Invitrogen, Burlington, Canada) for 3 times, then refilled with 4 mL fresh Opti-MEM prior to SECM experiments. Optical images were taken with an inverted microscopic lens (50×, Nikon, Japan). The resolution of SECM was about 5 μm because a 5.0 μm diameter Pt ultramicroelectrodes (UME) was used in the experiment. The SECM principle, instrumentation, operating procedures, and fabrication of 5 μm Pt UME were described in previous publications [10,12].


Time-lapse SECM is an ideal platform for monitoring real-time morphological change of live cells. With dissolve oxygen as the probing molecule, undesired artifacts caused by additive redox mediators are avoided, and the morphological change reflects actual natural response to the stimulation of interest (e.g. temperature stress). 5 μm diameter Pt UMEs provide adequate resolution to follow the dynamic morphological changes. The time-lapse SECM images presented in this paper possess remarkably high spatial resolution compared to 50 × optical microscopic images. Time-lapse cross section lines extracted from the time-lapse SECM images reveal specific local details of the dynamic topographical change. The cross section lines can be drawn from any position thus can be utilized to monitor the real-time topographical change of any interested local spot of a live cell. The acquisition time of each SECM image is only 3 min, which make it simple and convenient to film the movements of live cells.


SECM: Scanning electrochemical microscopy; UME: Ultramicroelectrode.

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

MMNZ carried out the experiment and drafted the manuscript. YTL prepared the manuscript for submission and coordinated final formulation, ZD formulated the research idea and prepared the manuscript draft version. All authors read and approved the final manuscript.

Supplementary Material Additional file 1

Dynamic morphology of a T24 cell under room temperature. temperature.

Click here for additional data file (1752-153X-6-20-S1.MOV)


This research was supported by NSERC Grant (Canada), the National Science Fund for Distinguished Young Scholars (21125522, China), the National Natural Science Foundation of China (91027035),the Fundamental Research Funds for the Central Universities (WK1013002, China) and the Open Project Program of the State Key Laboratory of Chemical Engineering (ECUST, SKL-ChE-11 C01, China). MMNZ was supported by the China Scholarship Council for her research in Canada.

Lepekhin EA,Walmod PS,Berezin A,Berezin V,Bock E,Evaluation of Cell MorphologyCytoskel Meth ProtocYear: 200016185100
Lippincott-Schwartz J,Patterson GH,Development and Use of Fluorescent Protein Markers in Living CellsScienceYear: 2003300879110.1126/science.108252012677058
Stephens DJ,Allan VJ,Light Microscopy Techniques for Live Cell ImagingScienceYear: 2003300828610.1126/science.108216012677057
You HX,Lau JM,Zhang S,Yu L,Atomic force microscopy imaging of living cells: a preliminary study of the disruptive effect of the cantilever tip on cell morphologyUltramicroscopyYear: 20008229730510.1016/S0304-3991(99)00139-410741682
Beaulieu I,Kuss S,Mauzeroll J,Geissler M,Biological Scanning Electrochemical Microscopy and Its Application to Live Cell StudiesAnal ChemYear: 2011831485149210.1021/ac101906a
Zhao X,Diakowski PM,Ding Z,Deconvoluting Topography and Spatial Physiological Activity of Live Macrophage Cells by Scanning Electrochemical Microscopy in Constant-Distance ModeAnal ChemYear: 2010828371837310.1021/ac101524v20873801
Bard AJ,Li X,Zhan W,Chemically imaging living cells by scanning electrochemical microscopyBiosens BioelectronYear: 20062246147210.1016/j.bios.2006.04.02816797958
Shigeru Amemiya AJB,Fan Fu-Ren F,Mirkin Michael V,Unwin Patrick R,Scanning Electrochemical MicroscopyAnnu Rev Anal ChemYear: 200819513110.1146/annurev.anchem.1.031207.112938
Bard AJ,Mirkin MV,Scanning Electrochemical MicroscopyYear: 2001New York: Mercel Dekker
Renkang Zhu ZD,Enhancing image quality of scanning electrochemical microscopy by improved probe fabrication and displacementCan J ChemYear: 2005831779179110.1139/v05-189
Amemiya S,Guo J,Xiong H,Gross D,Biological applications of scanning electrochemical microscopy: chemical imaging of single living cells and beyondAnal Bioanal ChemYear: 200638645847110.1007/s00216-006-0510-616855816
Zhao NOP X,Ding Z,Comparison study of live cells by atomic force microscopy, confocal microscopy, and scanning electrochemical microscopyCan J ChemYear: 20078517518310.1139/v07-007
Diakowski PM,Ding Z,Interrogation of living cells using alternating current scanning electrochemical microscopy (AC-SECM)Phys Chem Chem PhysYear: 200795966597418004408
Zhao X-C,Zhang M-N,Long Y-T,Ding Z-F,Redox reactions of reactive oxygen species in aqueous solutions as the probe for scanning electrochemical microscopy of single live T24 cellsCan J ChemYear: 20108856957610.1139/V10-051
Kamat AM,Lamm DL,Chemoprevention of urological cancerJ UrologyYear: 19991611748176010.1016/S0022-5347(05)68793-9
What you need to know about bladder cancer
Weinberg R,One Renegade Cell: The Quest For The Origin Of Cancer (Science Masters). Basic BooksYear: 1999
Templer RH,Ces O,New frontiers in single-cell analysisJ Roy Soc InterfaceYear: 20085S111S11210.1098/rsif.2008.0279.focus18664433
Zhao X,Lam S,Jass J,Ding Z,Scanning electrochemical microscopy of single human urinary bladder cells using reactive oxygen species as probe of inflammatory responseElectrochem CommYear: 20101277377610.1016/j.elecom.2010.03.030
Zhang MMN,Long Y-T,Ding Z,Cisplatin effects on evolution of reactive oxygen species from single human bladder cancer cells investigated by scanning electrochemical microscopyJ Inorg BiochemYear: 201110811512222169205
Mancuso S,Papeschi G,Marras AM,A polarographic, oxygen-selective, vibrating-microelectrode system for the spatial and temporal characterisation of transmembrane oxygen fluxes in plantsPlantaYear: 200021138438910.1007/s00425000029610987557
Bard AJ,Fan FRF,Kwak J,Lev O,Scanning electrochemical microscopy. Introduction and principlesAnal ChemYear: 19896113213810.1021/ac00177a011
Kwak J,Bard AJ,Scanning electrochemical microscopy. Theory of the feedback modeAnal ChemYear: 1989611221122710.1021/ac00186a009
Ndozangue-Touriguine O,Hamelin J,Bréard J,Cytoskeleton and apoptosisBiochem pharmacolYear: 200876111810.1016/j.bcp.2008.03.01618462707


[Figure ID: F1]
Figure 1 

Schematic graph showing the SECM setup for mapping the morphology of a T24 cell with dissolved oxygen as the redox mediator.

[Figure ID: F2]
Figure 2 

Comparison of the SECM images (a, b) and the optical micrographs (50 ×, c, d) of a T24 cell. The scanning area in SECM images was (a) 80 × 60 μm and (b) 80 × 80 μm. The acquisition time of each SECM image was about 3 min (256 × 256 pixels). The color profile of each image indicates the profile of the detected current in the scanning area. To keep all the images in proper contrast, every image uses its own color scale. The inset of Figure 2a is the cross section line extracted from the red dashed position in the SECM image. The background tilt was adjusted. In cross section lines, lower current indicates higher height of a certain spot.

[Figure ID: F3]
Figure 3 

Time-lapse SECM images presenting the changing morphology of a live T24 cell under room temperature taken with a 5 μm diameter Pt UME. The scanning area of each image was 80 × 60 μm (a-d)/80 × 80 μm (e-p). The acquisition time of each image was about 3 min (256 × 256 pixels). Time-lapse cross section lines extracted from Figure 3a-p present the changing topography of the T24 cell under the room temperature. Each cross section line goes through the center of the cell nucleus. Background tilt was adjusted. Red arrows: shrinking cellular membrane. Green triangles: non-nucleus part of the great nuclear area. Yellow triangles: nucleus part of the great nuclear area.

Article Categories:
  • Preliminary Communication

Keywords: Label-free, Scanning electrochemical microscopy, Dynamic morphology, Human bladder cancer cell.

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