| Filling a cavity dramatically increases pressure stability of the c-Myb R2 subdomain. | |
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MedLine Citation:
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PMID: 11536365 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cavities or packing defects in proteins may generally be related with the dynamics and function of a protein. In the c-Myb R2 subdomain, its single cavity has been shown to be crucial for its DNA recognition. Cavities are also considered important in determining the pressure stability of a protein. In the present work, high-pressure proton nuclear magnetic resonance ((1)H NMR) spectroscopy at 750 MHz is used to study the effect of a cavity-filling mutation (V103L) on the stability of the c-Myb R2 subdomain in the pressure range between 1 and 3,700 bar at 5 degrees C. A dramatic increase in the pressure stability of the c-Myb R2 subdomain is attained, from which we estimate the cavity size to be 35.3 A(3), in good agreement with literature values. We also evaluated the increase in thermodynamic stability DeltaG(0)(1bar) from 5.35 kJ/mol to 7.34 kJ/mol by the mutation, giving a clear example of the effect of a cavity on the global stability of a globular protein. |
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Authors:
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M W Lassalle; H Yamada; H Morii; K Ogata; A Sarai; K Akasaka |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Proteins Volume: 45 ISSN: 0887-3585 ISO Abbreviation: Proteins Publication Date: 2001 Oct |
Date Detail:
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Created Date: 2001-09-05 Completed Date: 2001-11-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8700181 Medline TA: Proteins Country: United States |
Other Details:
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Languages: eng Pagination: 96-101 Citation Subset: IM |
Copyright Information:
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Copyright 2001 Wiley-Liss, Inc. |
Affiliation:
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Department of Molecular Science, Graduate School of Science and Technology, Kobe University, Kobe, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Magnetic Resonance Spectroscopy Models, Molecular Mutation / genetics Pressure Protein Conformation Protein Denaturation Protein Folding Protein Renaturation Protein Structure, Tertiary Proto-Oncogene Proteins c-myb / chemistry*, genetics Thermodynamics |
| Chemical | |
Reg. No./Substance:
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0/Proto-Oncogene Proteins c-myb |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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