Document Detail

Filler particles used in dental biomaterials induce production and release of inflammatory mediators in vitro.
MedLine Citation:
PMID:  18759324     Owner:  NLM     Status:  MEDLINE    
Although dental composites are in extensive use today, little is known about the biological effects of the filler particles. As composite materials are gradually broken down in the aggressive environment of the oral cavity, the filler particles may leak and induce toxic effects on the surrounding tissue and cells. The aim of this study was to elucidate possible adverse biological effects of commonly used dental filler particles; bariumaluminiumsilica (BaAlSi) and bariumaluminiumfluorosilica (BaAlFSi) with mean size of 1 microm. BEAS-2B cells were used as a model system. Particle morphology, mean particle size in solution, and particle surface charge were determined by scanning electron microscopy and Malvern zetasizer technology, respectively. Enzyme-linked immunosorbent assay was used to detect secretion of cytokine and chemokine (IL-8 and IL-6) and quantitative PCR for detection of gene activity. Both types of particle increased the release of IL-6 and IL-8 in a dose-dependent manner. BaAlFSi particles induced a more marked IL-8 response compared to BaAlSi particles, whereas no significant difference was observed for the IL-6 response. Mechanistic studies using specific inhibitors and activators indicated that cyclic AMP-dependent protein kinase A is partly involved in the observed IL-8 response. In conclusion, we consider dental filler particles to have potential to induce adverse biological response in cell cultures.
Vibeke E Ansteinsson; Jan Tore Samuelsen; Jon E Dahl
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical materials research. Part B, Applied biomaterials     Volume:  89     ISSN:  1552-4981     ISO Abbreviation:  J. Biomed. Mater. Res. Part B Appl. Biomater.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-16     Completed Date:  2009-05-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234238     Medline TA:  J Biomed Mater Res B Appl Biomater     Country:  United States    
Other Details:
Languages:  eng     Pagination:  86-92     Citation Subset:  IM    
Nordic Institute of Dental Materials, Haslum, Norway.
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MeSH Terms
Aluminum Silicates / chemistry,  immunology
Barium Compounds / chemistry,  immunology
Biocompatible Materials / chemistry,  metabolism*
Cell Line
Cyclic AMP-Dependent Protein Kinases / metabolism
Dental Materials / chemistry,  metabolism*
Fluorides / chemistry,  immunology
Inflammation Mediators / chemistry,  immunology*
Interleukin-6 / genetics,  immunology
Interleukin-8 / genetics,  immunology
Materials Testing
Particle Size
RNA, Messenger / metabolism
Reg. No./Substance:
0/Aluminum Silicates; 0/Barium Compounds; 0/Biocompatible Materials; 0/Dental Materials; 0/Fluorides; 0/Inflammation Mediators; 0/Interleukin-6; 0/Interleukin-8; 0/RNA, Messenger; EC AMP-Dependent Protein Kinases

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