Document Detail

Filarial nematode parasites secrete a homologue of the human cytokine macrophage migration inhibitory factor.
MedLine Citation:
PMID:  9826378     Owner:  NLM     Status:  MEDLINE    
Filarial nematode parasites establish long-term chronic infections in the context of an antiparasite immunity that is strongly biased toward a Th2 response. The mechanisms that lead to this Th2 bias toward filarial antigens are not clear, but one possibility is that the parasites produce molecules that have the capacity to proactively modify their immunological environment. Here we report that filarial parasites of humans secrete a homologue of the human proinflammatory cytokine macrophage migration inhibitory factor (MIF) that has the capability of modifying the activity of human monocytes/macrophages. A cDNA clone isolated from a Brugia malayi infective-stage larva expression library encoded a 12.5-kDa protein product (Bm-MIF) with 42% identity to human and murine MIF. MIF homologues were also found to be expressed in the related filarial species Wuchereria bancrofti and Onchocerca volvulus. Bm-mif was transcribed by adult and larval parasites, and the protein product was found in somatic extracts and in the parasite's excretory-secretory products. Immunohistocytochemistry revealed that Bm-MIF was localized to cells of the hypodermis/lateral chord, the uterine wall, and larvae developing in utero. Unexpectedly, the activities of recombinant Bm-MIF and human MIF on human monocytes/macrophages were found to be similar. When placed with monocytes/macrophages in a cell migration assay, Bm-MIF inhibited random migration. When placed away from cells, Bm-MIF induced an increase in monocyte/macrophage migration that was specifically inhibited by neutralizing anti-Bm-MIF antibodies. Bm-MIF is the first demonstration that helminth parasites produce cytokine homologues that have the potential to modify host immune responses to promote parasite survival.
D V Pastrana; N Raghavan; P FitzGerald; S W Eisinger; C Metz; R Bucala; R P Schleimer; C Bickel; A L Scott
Related Documents :
357658 - Possible role of macrophage glycolipids as receptors for migration inhibitory factor (m...
12046088 - Evidences for vagus nerve in maintenance of immune balance and transmission of immune i...
10521368 - Vitamin c protects human vascular smooth muscle cells against apoptosis induced by mode...
22280238 - Concomitant gene mutations of mbl and cybb in chronic granulomatous disease: implicatio...
23106658 - Trichuris suis ova therapy for allergic rhinitis does not affect allergen-specific cyto...
12765338 - Structural basis for the proinflammatory cytokine activity of high mobility group box 1.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  66     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1998-12-24     Completed Date:  1998-12-24     Revised Date:  2014-06-20    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5955-63     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF040629;  U88035
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acid Sequence
Brugia malayi / genetics
Cell Movement
Filarioidea / genetics,  metabolism*
Macrophage Migration-Inhibitory Factors / genetics,  pharmacology,  secretion*
Macrophages / drug effects
Molecular Sequence Data
Monocytes / drug effects
Onchocerca volvulus / genetics
Sequence Homology, Amino Acid
Wuchereria bancrofti / genetics
Grant Support
Reg. No./Substance:
0/Macrophage Migration-Inhibitory Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Detection of anionic antimicrobial peptides in ovine bronchoalveolar lavage fluid and respiratory ep...
Next Document:  Local cytokine response in Helicobacter pylori-infected subjects.