Document Detail


Filamin A is a regulator of blood-testis barrier assembly during postnatal development in the rat testis.
MedLine Citation:
PMID:  22872576     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The blood-testis barrier (BTB) is an important ultrastructure in the testis. A delay in its assembly during postnatal development leads to meiotic arrest. Also, a disruption of the BTB by toxicants in adult rats leads to a failure in spermatogonial differentiation. However, the regulation of BTB assembly remains unknown. Herein, filamin A, an actin filament cross-linker that is known to maintain and regulate cytoskeleton structure and function in other epithelia, was shown to be highly expressed during the assembly of Sertoli cell BTB in vitro and postnatal development of BTB in vivo, perhaps being used to maintain the actin filament network at the BTB. A knockdown of filamin A by RNA interference was found to partially perturb the Sertoli cell tight junction (TJ) permeability barrier both in vitro and in vivo. Interestingly, this down-regulating effect on the TJ barrier function after the knockdown of filamin A was associated with a mis-localization of both TJ and basal ectoplasmic specialization proteins. Filamin A knockdown also induced a disorganization of the actin filament network in Sertoli cells in vitro and in vivo. Collectively, these findings illustrate that filamin A regulates BTB assembly by recruiting these proteins to the microenvironment in the seminiferous epithelium to serve as the building blocks. In short, filamin A participates in BTB assembly by regulating protein recruitment during postnatal development in the rat testis.
Authors:
Wenhui Su; Dolores D Mruk; Pearl P Y Lie; Wing-Yee Lui; C Yan Cheng
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-08-07
Journal Detail:
Title:  Endocrinology     Volume:  153     ISSN:  1945-7170     ISO Abbreviation:  Endocrinology     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-24     Completed Date:  2012-12-11     Revised Date:  2013-10-10    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5023-35     Citation Subset:  AIM; IM    
Affiliation:
Population Council, New York, New York 10065, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood-Testis Barrier / growth & development,  metabolism*
Cells, Cultured
Contractile Proteins / genetics,  metabolism*
Male
Microfilament Proteins / genetics,  metabolism*
Permeability
RNA Interference
Rats
Rats, Sprague-Dawley
Sertoli Cells / cytology,  metabolism*
Testis / cytology,  growth & development,  metabolism*
Tight Junctions / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
R01 HD056034/HD/NICHD NIH HHS; U54 HD029990/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Contractile Proteins; 0/Microfilament Proteins; 0/filamins
Comments/Corrections

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