| Filamin A is a regulator of blood-testis barrier assembly during postnatal development in the rat testis. | |
| | |
MedLine Citation:
|
PMID: 22872576 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The blood-testis barrier (BTB) is an important ultrastructure in the testis. A delay in its assembly during postnatal development leads to meiotic arrest. Also, a disruption of the BTB by toxicants in adult rats leads to a failure in spermatogonial differentiation. However, the regulation of BTB assembly remains unknown. Herein, filamin A, an actin filament cross-linker that is known to maintain and regulate cytoskeleton structure and function in other epithelia, was shown to be highly expressed during the assembly of Sertoli cell BTB in vitro and postnatal development of BTB in vivo, perhaps being used to maintain the actin filament network at the BTB. A knockdown of filamin A by RNA interference was found to partially perturb the Sertoli cell tight junction (TJ) permeability barrier both in vitro and in vivo. Interestingly, this down-regulating effect on the TJ barrier function after the knockdown of filamin A was associated with a mis-localization of both TJ and basal ectoplasmic specialization proteins. Filamin A knockdown also induced a disorganization of the actin filament network in Sertoli cells in vitro and in vivo. Collectively, these findings illustrate that filamin A regulates BTB assembly by recruiting these proteins to the microenvironment in the seminiferous epithelium to serve as the building blocks. In short, filamin A participates in BTB assembly by regulating protein recruitment during postnatal development in the rat testis. |
| | |
Authors:
|
Wenhui Su; Dolores D Mruk; Pearl P Y Lie; Wing-Yee Lui; C Yan Cheng |
Related Documents
:
|
9495556 - Cytokine-induced neutrophil chemoattractant gene expression in the rat hypothalamus by ... 20214896 - Cb(1) modulation of hormone secretion, neuronal activation and mrna expression followin... 3194396 - Pituitary-specific expression and glucocorticoid regulation of a proopiomelanocortin fu... 15787816 - Corticotropin-releasing hormone triggers differentiation in hacat keratinocytes. 12750006 - L-type high voltage-gated calcium channels cause an increase in diazepam binding inhibi... 12723986 - Functional analysis of an arthritogenic synovial fibroblast. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-08-07 |
Journal Detail:
|
Title: Endocrinology Volume: 153 ISSN: 1945-7170 ISO Abbreviation: Endocrinology Publication Date: 2012 Oct |
Date Detail:
|
Created Date: 2012-09-24 Completed Date: 2012-12-11 Revised Date: 2013-04-16 |
Medline Journal Info:
|
Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
|
Languages: eng Pagination: 5023-35 Citation Subset: AIM; IM |
Affiliation:
|
Population Council, New York, New York 10065, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blood-Testis Barrier / growth & development, metabolism* Cells, Cultured Contractile Proteins / genetics, metabolism* Male Microfilament Proteins / genetics, metabolism* Permeability RNA Interference Rats Rats, Sprague-Dawley Sertoli Cells / cytology, metabolism* Testis / cytology, growth & development, metabolism* Tight Junctions / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
R01 HD056034/HD/NICHD NIH HHS; U54 HD029990/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Contractile Proteins; 0/Microfilament Proteins; 0/filamins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Phase I Studies of Sirolimus Alone or in Combination with Pharmacokinetic Modulators in Advanced Can...
Next Document: Mullerian Inhibiting Substance Recruits ALK3 to Regulate Leydig Cell Differentiation.