Document Detail

Fifteen weeks of dietary n-3 polyunsaturated fatty acid deprivation increase turnover of n-6 docosapentaenoic acid in rat-brain phospholipids.
MedLine Citation:
PMID:  22142872     Owner:  NLM     Status:  MEDLINE    
Docosapentaenoic acid (DPAn-6, 22:5n-6) is an n-6 polyunsaturated fatty acid (PUFA) whose brain concentration can be increased in rodents by dietary n-3 PUFA deficiency, which may contribute to their behavioral dysfunction. We used our in vivo intravenous infusion method to see if brain DPAn-6 turnover and metabolism also were altered with deprivation. We studied male rats that had been fed for 15 weeks post-weaning an n-3 PUFA adequate diet containing 4.6% alpha-linolenic acid (α-LNA, 18:3n-3) or a deficient diet (0.2% α-LNA), each lacking docosahexaenoic acid (22:6n-3) and arachidonic acid (AA, 20:4n-6). [1-(14)C]DPAn-6 was infused intravenously for 5min in unanesthetized rats, after which the brain underwent high-energy microwaving, and then was analyzed. The n-3 PUFA deficient compared with adequate diet increased DPAn-6 and decreased DHA concentrations in plasma and brain, while minimally changing brain AA concentration. Incorporation rates of unesterified DPAn-6 from plasma into individual brain phospholipids were increased 5.2-7.7 fold, while turnover rates were increased 2.1-4.7 fold. The observations suggest that increased metabolism and brain concentrations of DPAn-6 and its metabolites, together with a reduced brain DHA concentration, contribute to behavioral and functional abnormalities reported with dietary n-3 PUFA deprivation in rodents. (196 words).
Miki Igarashi; Hyung-Wook Kim; Fei Gao; Lisa Chang; Kaizong Ma; Stanley I Rapoport
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural     Date:  2011-11-30
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1821     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-07-16     Completed Date:  2013-02-15     Revised Date:  2013-09-03    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1235-43     Citation Subset:  IM    
Copyright Information:
Published by Elsevier B.V.
National Institute on Aging, National Institutes of Health, Bethesda, MD, USA.
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MeSH Terms
Brain / metabolism*
Brain Chemistry
Fatty Acids, Omega-3*
Fatty Acids, Unsaturated / metabolism*
Phospholipids / immunology*
Rats, Inbred F344
Time Factors
Grant Support
Reg. No./Substance:
0/Fatty Acids, Omega-3; 0/Fatty Acids, Unsaturated; 0/Phospholipids; 25448-00-4/docosapentaenoic acid

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