Document Detail

Fiducial points for three-dimensional computer-assisted reconstruction of serial light microscopic sections of umbilical cord.
MedLine Citation:
PMID:  2012317     Owner:  NLM     Status:  MEDLINE    
Fascicles of human sural (peripheral sensory) nerve were used as external fiducial points (FP) for accurate registration of serial light microscopic sections in three-dimensional reconstruction of human umbilical cord. This paper describes a method for embedding the FPs within the paraffin wax block simultaneously with the specimen to be sectioned. Using a new design of embedding box, the FPs are embedded close to the specimen and are transferred to the slides as part of the tissue sections. Three to four FPs were used to align and scale the serial tissue sections for digitization and computerized reconstruction, using a commercially available software program on IBM-compatible 80386 hardware. A three-dimensional solid surface graphic model of a segment of human umbilical cord was generated.
I Ongaro; G H Sperber; G A Machin; C A Murdoch
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Anatomical record     Volume:  229     ISSN:  0003-276X     ISO Abbreviation:  Anat. Rec.     Publication Date:  1991 Feb 
Date Detail:
Created Date:  1991-05-07     Completed Date:  1991-05-07     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0370540     Medline TA:  Anat Rec     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  285-9     Citation Subset:  IM    
Department of Oral Biology, Faculty of Dentistry University of Alberta, Edmonton, Canada.
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MeSH Terms
Histological Techniques
Image Processing, Computer-Assisted / instrumentation,  methods*
Umbilical Cord / anatomy & histology*,  blood supply

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