Document Detail


Fibrosis, not cell size, delineates beta-myosin heavy chain reexpression during cardiac hypertrophy and normal aging in vivo.
MedLine Citation:
PMID:  17068123     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reexpression of the fetally expressed beta-myosin heavy chain (beta-MHC) gene is a well documented marker of pathological cardiac hypertrophy and normal aging in many experimental models. To gain insights into factors affecting this reexpression of beta-MHC within the complex anatomical structure of the heart, we investigated the spatial pattern of its expression at the level of single cells during aging and hypertrophy. We generated mice that express yellow fluorescent protein fused to the N terminus of the beta-MHC and examined its expression pattern during normal aging and in mice with hypertrophy induced by constitutive expression of a renin transgene. The localization of fibrosis within the hearts also was determined by using a fluorescent lectin. The results show that reexpression of beta-MHC occurs in discrete subsets of myocytes within the subendocardium rather than uniformly throughout the heart, that beta-MHC induction is not an obligatory consequence of cellular hypertrophy, and that beta-MHC-expressing cells in the normal aging heart and the hypertrophic heart are distributed predominantly in clusters within and surrounding foci of fibrosis. We conclude that beta-MHC gene expression in the normal aging adult and hypertrophic mouse heart is a marker of fibrosis rather than of cellular hypertrophy.
Authors:
Kumar Pandya; Hyung-Suk Kim; Oliver Smithies
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-10-26
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  103     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-08     Completed Date:  2006-12-20     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  16864-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aging / genetics*,  pathology*
Animals
Animals, Newborn
Bacterial Proteins / genetics
Cardiomegaly / genetics*,  pathology*
Cell Enlargement
Cell Size
Female
Fibrosis
Gene Expression Regulation, Developmental
Luminescent Proteins / genetics
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Myocytes, Cardiac / metabolism,  pathology
Myosin Heavy Chains / genetics*
Ventricular Myosins / genetics*
Grant Support
ID/Acronym/Agency:
HL 48277/HL/NHLBI NIH HHS; HL 71266/HL/NHLBI NIH HHS; R01 HL049277/HL/NHLBI NIH HHS; R01 HL049277-14/HL/NHLBI NIH HHS; R01 HL049277-15/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Luminescent Proteins; 0/yellow fluorescent protein, Bacteria; EC 3.6.1.-/Ventricular Myosins; EC 3.6.4.1/Myosin Heavy Chains
Comments/Corrections

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