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Fibronectin influences cellular proliferation and apoptosis similarly in LNCaP and PC-3 prostate cancer cell lines.
MedLine Citation:
PMID:  10869957     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The mechanisms responsible for the emergence of clinically advanced prostate cancer (PC) are incompletely understood. Recent studies suggest that altered tumoral apoptosis with disordered cell proliferation sustains advanced disease and may account for the phenomena of anti-androgen therapeutic resistance. Previous inquiry has focused primarily on faulty intracellular mechanisms with limited scrutiny of the extracellular matrix including fibronectin and collagen type 4. We evaluated cell proliferation with Ki-67 immunoassay/image analysis and apoptosis by TUNEL staining and Bcl-2 immunoassay/image analysis in LNCaP and PC-3 human PC cell lines at baseline and following propagation on fibronectin and collagen type 4-coated coverslip substrate. Cell cultures showed differing proliferative and apoptosis characteristics at baseline, with the LNCaP cell line showing relatively higher proliferation and apoptosis rates than the PC-3 cell line. Cell proliferation and apoptosis were statistically significantly decreased in both cell lines following propagation on fibronectin. Bcl-2 expression was significantly increased among both cell lines following propagation on fibronectin. In contrast, cell proliferation, apoptosis, and Bcl-2 expression showed insignificant changes in both cell lines following uncoated coverslip and collagen type 4 matrix propagation. Our findings showed that fibronectin influences cell proliferation, apoptosis, and Bcl-2 expression similarly among LNCaP and PC-3 PC cell lines. It is likely that the altered rates are independent of the androgen status of the cell line and are mediated through a nonhormonal mechanism.
Authors:
Morgan; Saba; Gower
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Urologic oncology     Volume:  5     ISSN:  1873-2496     ISO Abbreviation:  Urol. Oncol.     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-06-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9805460     Medline TA:  Urol Oncol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  155-159     Citation Subset:  -    
Affiliation:
Department of Pathology University of South Florida College Medicine, James Haley VA Hospital, 13000 Bruce B. Downs Blvd., 33612, Tampa, FL, USA
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