Document Detail

Fibronectin-hepatocyte growth factor enhances reendothelialization in tissue-engineered heart valve.
MedLine Citation:
PMID:  16242457     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: To overcome the limitations of tissue-engineered heart valves, which require cell seeding before implantation, a growth factor for in situ recellularization may be an important strategy. We developed a new decellularized valve containing a fusion protein combined fibronectin and hepatocyte growth factor. Here, we tested the hypothesis that our valve might accelerate in situ recellularization by inducing the proliferation of endothelial cells. METHODS: Porcine aortic valves were decellularized using detergent. Fibronectin-hepatocyte growth factor was introduced into the decellularized valves. The decellularized valves with fibronectin-hepatocyte growth factor were implanted into the pulmonary arterial trunk of dogs (F group: n = 15). As controls, decellularized valves without the growth factor (C group: n = 12), and with hepatocyte growth factor (H group: n = 12) were implanted in the same manner. Histologic examinations were performed 1 week and 1 month after implantation. RESULTS: One week after implantation, endothelial cells partially covered the surface of the graft in the F group but not the C and H groups. Although the C and H groups had inadequate recellularization 1 month after implantation, the F group showed a monolayer of endothelial cells, underneath which were areas of additional cell layers, which were vimentin positive. Quantitative evaluation demonstrated the amount of vimentin in the F group was 71% of the native control, and it was much lower in the other groups (C, 2.8%; H, 16.8%) 1 month after implantation. CONCLUSIONS: This study demonstrated that fibronectin-hepatocyte growth factor enhanced early in situ recellularization in decellularized valves.
Takeyoshi Ota; Yoshiki Sawa; Shigemitsu Iwai; Takashi Kitajima; Yuichiro Ueda; Chris Coppin; Hikaru Matsuda; Yutaka Okita
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Annals of thoracic surgery     Volume:  80     ISSN:  1552-6259     ISO Abbreviation:  Ann. Thorac. Surg.     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-10-24     Completed Date:  2006-11-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  15030100R     Medline TA:  Ann Thorac Surg     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1794-801     Citation Subset:  AIM; IM    
Department of Cardiovascular Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
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MeSH Terms
Aortic Valve / drug effects,  transplantation
Endothelium, Vascular / drug effects*
Fibronectins / pharmacology*
Hepatocyte Growth Factor / pharmacology*
Tissue Engineering / methods*
Reg. No./Substance:
0/Fibronectins; 67256-21-7/Hepatocyte Growth Factor
Comment In:
Ann Thorac Surg. 2005 Nov;80(5):1801-2   [PMID:  16242458 ]

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