| Fibronectin-hepatocyte growth factor enhances reendothelialization in tissue-engineered heart valve. | |
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MedLine Citation:
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PMID: 16242457 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: To overcome the limitations of tissue-engineered heart valves, which require cell seeding before implantation, a growth factor for in situ recellularization may be an important strategy. We developed a new decellularized valve containing a fusion protein combined fibronectin and hepatocyte growth factor. Here, we tested the hypothesis that our valve might accelerate in situ recellularization by inducing the proliferation of endothelial cells. METHODS: Porcine aortic valves were decellularized using detergent. Fibronectin-hepatocyte growth factor was introduced into the decellularized valves. The decellularized valves with fibronectin-hepatocyte growth factor were implanted into the pulmonary arterial trunk of dogs (F group: n = 15). As controls, decellularized valves without the growth factor (C group: n = 12), and with hepatocyte growth factor (H group: n = 12) were implanted in the same manner. Histologic examinations were performed 1 week and 1 month after implantation. RESULTS: One week after implantation, endothelial cells partially covered the surface of the graft in the F group but not the C and H groups. Although the C and H groups had inadequate recellularization 1 month after implantation, the F group showed a monolayer of endothelial cells, underneath which were areas of additional cell layers, which were vimentin positive. Quantitative evaluation demonstrated the amount of vimentin in the F group was 71% of the native control, and it was much lower in the other groups (C, 2.8%; H, 16.8%) 1 month after implantation. CONCLUSIONS: This study demonstrated that fibronectin-hepatocyte growth factor enhanced early in situ recellularization in decellularized valves. |
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Authors:
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Takeyoshi Ota; Yoshiki Sawa; Shigemitsu Iwai; Takashi Kitajima; Yuichiro Ueda; Chris Coppin; Hikaru Matsuda; Yutaka Okita |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Annals of thoracic surgery Volume: 80 ISSN: 1552-6259 ISO Abbreviation: Ann. Thorac. Surg. Publication Date: 2005 Nov |
Date Detail:
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Created Date: 2005-10-24 Completed Date: 2006-11-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 15030100R Medline TA: Ann Thorac Surg Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1794-801 Citation Subset: AIM; IM |
Affiliation:
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Department of Cardiovascular Surgery, Kobe University Graduate School of Medicine, Kobe, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aortic Valve / drug effects, transplantation Dogs Endothelium, Vascular / drug effects* Fibronectins / pharmacology* Hepatocyte Growth Factor / pharmacology* Swine Tissue Engineering / methods* |
| Chemical | |
Reg. No./Substance:
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0/Fibronectins; 67256-21-7/Hepatocyte Growth Factor |
| Comments/Corrections | |
Comment In:
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Ann Thorac Surg. 2005 Nov;80(5):1801-2
[PMID:
16242458
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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