| Fibronectin and asialoglyprotein receptor mediate hepatitis B surface antigen binding to the cell surface. | |
| | |
MedLine Citation:
|
PMID: 20364278 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Both fibronectin and the asialoglycoprotein receptor (ASGPR) have been identified by some investigators as partners for hepatitis B virus (HBV) envelope proteins. Because fibronectin is a natural ligand for ASGPR, we speculated that HBV might attach to ASGPR expressed on the hepatocyte surface via fibronectin. To test this hypothesis, we first confirmed by co-immunoprecipitation that ASGPR, fibronectin and HBsAg bind to each other in HepG2.2.15 cells, and possible binding domains were identified by GST pull-down. In addition, by measuring binding of HBsAg to cells, we found that ASGPR and fibronectin enhanced the binding capability of HBsAg to HepG2 cells, and even to 293T and CHO cells, which normally do not bind HBV. In conclusion, our findings suggest that both fibronectin and ASGPR mediate HBsAg binding to the cell surface, which provides further evidence for the potential roles of these two proteins in mediating HBV binding to liver cells. |
| | |
Authors:
|
Jing Yang; Feng Wang; Linlin Tian; Jing Su; Xiangqian Zhu; Li Lin; Xiaoran Ding; Xuejun Wang; Shengqi Wang |
Related Documents
:
|
2548598 - Conformational changes of plasma fibronectin detected upon adsorption to solid substrat... 1544908 - Binding of the proteoglycan decorin to collagen type vi. 1901868 - Laminin fragment e8 mediates pc12 cell neurite outgrowth by binding to cell surface bet... 11416848 - Mapping critical sites in collagen ii for rational design of gene-engineered proteins f... 15698958 - Ligand-binding specificity of an invertebrate (manduca sexta) putative cellular retinoi... 16238298 - Diastereomeric molecular recognition and binding behavior of bile acids by l/d-tryptoph... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-03 |
Journal Detail:
|
Title: Archives of virology Volume: 155 ISSN: 1432-8798 ISO Abbreviation: Arch. Virol. Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-06-03 Completed Date: 2010-07-08 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7506870 Medline TA: Arch Virol Country: Austria |
Other Details:
|
Languages: eng Pagination: 881-8 Citation Subset: IM |
Affiliation:
|
Beijing Institute of Radiation Medicine, 27 Taiping Road, Haidian District, Beijing, People's Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Asialoglycoprotein Receptor / metabolism* CHO Cells Cell Line Cricetinae Cricetulus Fibronectins / metabolism* Hep G2 Cells Hepatitis B Surface Antigens / metabolism* Hepatitis B virus / metabolism, pathogenicity* Hepatocytes / metabolism, virology* Humans Immunoprecipitation Receptors, Virus / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Asialoglycoprotein Receptor; 0/Fibronectins; 0/Hepatitis B Surface Antigens; 0/Receptors, Virus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The long-term functional outcome of type II odontoid fractures managed non-operatively.
Next Document: Lactic acid bacterial cell factories for gamma-aminobutyric acid.