Document Detail

Fibronectin Assembly in the Crypts of Cytokinesis-Blocked Multilobular Cells Promotes Anchorage-Independent Growth.
MedLine Citation:
PMID:  23951336     Owner:  NLM     Status:  In-Data-Review    
Anchorage-independent growth is a characteristic feature of cancer cells. However, it is unclear whether it represents a cause or a consequence of tumorigenesis. For normal cells, integrin-mediated adhesion is required for completion of the G1 and cytokinesis stages of the cell cycle. This study identified a mechanism that can drive anchorage-independent growth if the G1 checkpoint is suppressed. Cells with defective G1 checkpoint progressed through several rounds of the cell cycle in suspension in spite of uncompleted cytokinesis, thereby forming bi- and multilobular cells. Aurora B and CEP55 were localized to midbodies between the lobes, suggesting that the cytokinesis process reached close to abscission. Integrin-mediated re-attachment of such cells induced cytokinesis completion uncoupled from karyokinesis in most cells. However, a portion of the cells instead lost the constriction and became binucleated. Also, long-term suspension culture in soft agar produced colonies where the cytokinesis block was overcome. This process was fibronectin-dependent since fibronectin-deficient cells did not form colonies unless fibronectin was expressed or exogenously added. While fibronectin normally is not deposited on non-adherent single cells, bi/multilobular cells accumulated fibronectin in the intussusceptions. Based on our data we conclude: 1) Suppression of the G1 checkpoint allows multiple rounds of the cell cycle in detached cells and thereby enables matrix formation on their surface. 2) Uncompleted cytokinesis due to cell detachment resumes if integrin interactions are re-formed, allowing colony formation in soft agar 3) Such delayed cell division can generate binucleated cells, a feature known to cause chromosomal instability.
Rajesh Kumar Gupta; Staffan Johansson
Related Documents :
23291606 - The protective role of quince leaf extract against the adverse impacts of ultraviolet-a...
23676966 - Mechanisms of action of external volume expansion devices.
24840226 - Exploration on mechanism of a new type of melatonin receptor agonist neu-p11 in hypoxia...
24959246 - Inhibition of microrna-21 upregulates the expression of programmed cell death 4 and pho...
2828086 - Regulation of intracellular ph during the g1/s-phase transition of the neuroblastoma ce...
17896796 - Protective effect of prion protein via the n-terminal region in mediating a protective ...
Publication Detail:
Type:  Journal Article     Date:  2013-08-12
Journal Detail:
Title:  PloS one     Volume:  8     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2013  
Date Detail:
Created Date:  2013-08-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e72933     Citation Subset:  IM    
Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Sleep architecture when sleeping at an unusual circadian time and associations with insulin sensitiv...
Next Document:  Atomic Force Microscopy Analysis of the Role of Major DNA-Binding Proteins in Organization of the Nu...