Document Detail


Fibronectin-Alginate microcapsules improve cell viability and protein secretion of encapsulated Factor IX-engineered human mesenchymal stromal cells.
MedLine Citation:
PMID:  24564349     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Continuous delivery of proteins by engineered cells encapsulated in biocompatible polymeric microcapsules is of considerable therapeutic potential. However, this technology has not lived up to expectations due to inadequate cell-matrix interactions and subsequent cell death. In this study we hypothesize that the presence of fibronectin in an alginate matrix may enhance the viability and functionality of encapsulated human cord blood-derived mesenchymal stromal cells (MSCs) expressing the human Factor IX (FIX) gene. MSCs were encapsulated in alginate-PLL microcapsules containing 10, 100, or 500 μg/ml fibronectin to ameliorate cell survival. MSCs in microcapsules with 100 and 500 μg/ml fibronectin demonstrated improved cell viability and proliferation and higher FIX secretion compared to MSCs in non-supplemented microcapsules. In contrast, 10 μg/ml fibronectin did not significantly affect the viability and protein secretion from the encapsulated cells. Differentiation studies demonstrated osteogenic (but not chondrogenic or adipogenic) differentiation capability and efficient FIX secretion of the enclosed MSCs in the fibronectin-alginate suspension culture. Thus, the use of recombinant MSCs encapsulated in fibronectin-alginate microcapsules in basal or osteogenic cultures may be of practical use in the treatment of hemophilia B.
Authors:
Bahareh Sayyar; Megan Dodd; Leah Marquez-Curtis; Anna Janowska-Wieczorek; Gonzalo Hortelano
Related Documents :
24987429 - Effect of selenium-enriched garlic oil against cytotoxicity induced by ox-ldl in endoth...
23036999 - Lectin from agaricus bisporus inhibited s phase cell population and akt phosphorylation...
23260329 - The histomorphologic spectrum of spindle cell thymoma.
23229819 - P63 regulates cell proliferation and cell cycle progression‑associated genes in stromal...
22965909 - Crumbs limits oxidase-dependent signaling to maintain epithelial integrity and prevent ...
23739749 - Field-deployable whole-cell bioluminescent biosensors: so near and yet so far.
12533429 - Expression of wilms' tumor gene and protein localization during ovarian formation and f...
21445039 - Clonogenic assay: adherent cells.
22886539 - Retinoic acid derived from the fetal ovary initiates meiosis in mouse germ cells.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-2-25
Journal Detail:
Title:  Artificial cells, nanomedicine, and biotechnology (Print)     Volume:  -     ISSN:  2169-141X     ISO Abbreviation:  Artif Cells Nanomed Biotechnol     Publication Date:  2014 Feb 
Date Detail:
Created Date:  2014-2-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101594777     Medline TA:  Artif Cells Nanomed Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Nanotechnology: A magic bullet for HIV AIDS treatment.
Next Document:  Vesicular system: Versatile carrier for transdermal delivery of bioactives.