Document Detail


Fibroblasts isolated from human pterygia exhibit transformed cell characteristics.
MedLine Citation:
PMID:  7520809     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pterygium is a degenerative corneal limbal process and UV irradiation has been suggested as being a major environmental predisposing factor. The invasive nature of the fibroblasts associated with pterygia raises the question as to whether these cells are transformed. To test this hypothesis, we established fibroblast strains from autologous and heterologous pterygial and conjunctival specimens, respectively, from subjects between 40 to 50 yr of age, and compared their growth characteristics in culture. All pterygial fibroblast strains exhibited a reduced dependence on serum and exogenous growth factors for growth and reached a saturation population density that was threefold higher than conjunctival fibroblasts cultured under the same conditions. In addition, all pterygial fibroblast strains were able to form colonies in soft agar in 5% fetal bovine serum at a 6.0 to 7.5% efficiency. Under the same experimental conditions, none of the conjunctival fibroblast strains were able to grow. The results presented support the conclusion that pterygial fibroblasts have acquired many of the properties of the transformed phenotype.
Authors:
J K Chen; R J Tsai; S S Lin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  30A     ISSN:  1071-2690     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-09-29     Completed Date:  1994-09-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  243-8     Citation Subset:  IM    
Affiliation:
Department of Physiology, Chang Gung Medical College, Taoyuan, Taiwan, Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Adult
Cell Adhesion / drug effects
Cell Division / drug effects
Cell Line, Transformed
Conjunctiva / pathology
Epidermal Growth Factor / pharmacology
Fibroblast Growth Factor 1 / pharmacology
Fibroblasts / drug effects,  pathology*
Humans
Male
Middle Aged
Phenotype
Pterygium / pathology*
Time Factors
Transforming Growth Factor beta / pharmacology
Chemical
Reg. No./Substance:
0/Transforming Growth Factor beta; 104781-85-3/Fibroblast Growth Factor 1; 62229-50-9/Epidermal Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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