Document Detail

Fibroblastic cell cycling in collagen gels.
MedLine Citation:
PMID:  8439586     Owner:  NLM     Status:  MEDLINE    
Quiescent C3H10T1/2 mouse fibroblasts resume DNA synthesis and proliferation following incubation in medium containing fresh serum both when grown in monolayer and when grown in a collagen matrix. We observed that the rate of DNA synthesis is reduced at high initial cell densities and low initial collagen concentrations. In a collagen matrix, fibroblasts contract the matrix causing an increase in cell density and collagen concentration. We studied the chronological relationship between the kinetics of DNA synthesis and the collagen matrix contraction. The rate of collagen collection per cell changes in time, dependent on initial cell and collagen concentration. The kinetics of the collagen collection showed a positive correlation with the kinetics of DNA synthesis, 16 h later.
J E Souren; R van Wijk
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell proliferation     Volume:  26     ISSN:  0960-7722     ISO Abbreviation:  Cell Prolif.     Publication Date:  1993 Jan 
Date Detail:
Created Date:  1993-03-31     Completed Date:  1993-03-31     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9105195     Medline TA:  Cell Prolif     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  13-23     Citation Subset:  IM    
Department of Molecular Cell Biology, University of Utrecht, The Netherlands.
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MeSH Terms
Cell Adhesion
Cell Count
Cell Cycle*
Cells, Cultured
DNA / biosynthesis
Mice, Inbred C3H
Reg. No./Substance:
0/Actins; 0/Gels; 3326-32-7/Fluorescein-5-isothiocyanate; 9007-34-5/Collagen; 9007-49-2/DNA

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