Document Detail


Fibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors and during embryonic stem cell differentiation.
MedLine Citation:
PMID:  18504136     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neural stem (NS) cell lines may be derived via differentiation of pluripotent embryonic stem (ES) cells or from foetal forebrain. However, because NS cells arise in vitro from heterogeneous populations their immediate cellular origin remains unclear. We used microarray-based expression profiling to identify a set of markers expressed by mouse NS cells but not ES cells. One differentially expressed gene encodes the cell surface protein, CD44. CD44 expression is activated by FGF-2 in a subset of cells in both differentiating ES cells and foetal forebrain cultures. Following isolation by flow cytometry the CD44+ population was found to be highly enriched for NS cell founders. We found that other NS cell marker genes are also induced by FGF in culture, including: Adam12, Cadherin20, Cx3cl1, EGFR, Frizzled9, Kitl, Olig1, Olig2 and Vav3. We speculate that the self-renewing NS cell state may be generated in vitro following transcriptional resetting induced by FGF.
Authors:
Steven M Pollard; Richard Wallbank; Simon Tomlinson; Lars Grotewold; Austin Smith
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-10
Journal Detail:
Title:  Molecular and cellular neurosciences     Volume:  38     ISSN:  1095-9327     ISO Abbreviation:  Mol. Cell. Neurosci.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-30     Completed Date:  2008-12-09     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  9100095     Medline TA:  Mol Cell Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  393-403     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation* / drug effects,  physiology
Cell Line
Cells, Cultured
Embryonic Stem Cells / cytology*,  drug effects,  physiology
Female
Fibroblast Growth Factors / pharmacology*
Genetic Markers / drug effects,  physiology
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Neurons / cytology*,  drug effects,  physiology
Phenotype*
Pregnancy
Prosencephalon / cytology*,  drug effects,  physiology
Grant Support
ID/Acronym/Agency:
G0800784//Medical Research Council; G9806702//Medical Research Council; //Biotechnology and Biological Sciences Research Council
Chemical
Reg. No./Substance:
0/Genetic Markers; 62031-54-3/Fibroblast Growth Factors

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