Document Detail


Fibroblast growth factor-9 is an endometrial stromal growth factor.
MedLine Citation:
PMID:  12072406     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fibroblast growth factor-9 (FGF-9) is an autocrine/paracrine growth factor considered to be important for the growth and survival of motorneurons and prostate. In this study, we found that FGF-9 was expressed at high levels in normal uterine endometrium, especially during the late proliferative phase, which is coincident with the rise of estradiol and the time of uterine endometrial proliferation. Using quantitative RT-PCR analysis, we found that FGF-9 mRNA was expressed primarily by endometrial stromal cells. High affinity receptors of FGF-9 were detected in both epithelial and stromal cells but with distinct patterns. FGFR2IIIc and FGFR3IIIc are abundant in endometrial stromal cell. FGFR2IIIb is mostly expressed in endometrial epithelial cells, whereas FGFR3IIIb is found in both epithelial and stromal cells. Treatment with FGF-9 induces endometrial stromal proliferation in a dose-dependent manner. Expression of FGF-9 in stromal cells was induced by 17beta-estradiol but not by progesterone. Furthermore, the administration of 17beta-estradiol stimulates endometrial stromal cell proliferation and that can be inhibited by cotreatment with anti-FGF-9 antibody. Herein we demonstrate, for the first time, that FGF-9 is an autocrine estromedin endometrial stromal growth factor that plays roles in cyclic proliferation of uterine endometrial stroma.
Authors:
Shaw-Jenq Tsai; Meng-Hsing Wu; Hsiu-Mei Chen; Pei-Chin Chuang; Lih-Yuh C Wing
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrinology     Volume:  143     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-06-19     Completed Date:  2002-07-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2715-21     Citation Subset:  AIM; IM    
Affiliation:
Department of Physiology, National Cheng Kung University Medical College, Tainan 70101, Taiwan, Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Cell Division / physiology
Endometrium / cytology,  physiology*
Estradiol / pharmacology
Female
Fibroblast Growth Factor 9
Fibroblast Growth Factors / biosynthesis,  genetics,  physiology*
Gene Expression Regulation / drug effects
Growth Substances / physiology*
Humans
Immunohistochemistry
Ovary / physiology
Precipitin Tests
Progesterone / pharmacology
RNA, Messenger / biosynthesis,  genetics
Receptors, Fibroblast Growth Factor / biosynthesis,  genetics
Reverse Transcriptase Polymerase Chain Reaction
Stromal Cells / physiology*
Chemical
Reg. No./Substance:
0/FGF9 protein, human; 0/Fibroblast Growth Factor 9; 0/Growth Substances; 0/RNA, Messenger; 0/Receptors, Fibroblast Growth Factor; 50-28-2/Estradiol; 57-83-0/Progesterone; 62031-54-3/Fibroblast Growth Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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