Document Detail

Fibroblast growth factor 8 increases breast cancer cell growth by promoting cell cycle progression and by protecting against cell death.
MedLine Citation:
PMID:  19962979     Owner:  NLM     Status:  MEDLINE    
Fibroblast growth factor 8 (FGF-8) is expressed in a large proportion of breast cancers, whereas its level in normal mammary gland epithelium is low. Previous studies have shown that FGF-8b stimulates breast cancer cell growth in vitro and in vivo. To explore the mechanisms by which FGF-8b promotes growth, we studied its effects on cell cycle regulatory proteins and signalling pathways in mouse S115 and human MCF-7 breast cancer cells. We also studied the effect of FGF-8b on cell survival. FGF-8b induced cell cycle progression and up-regulated particularly cyclin D1 mRNA and protein in S115 cells. Silencing cyclin D1 with siRNA inhibited most but not all FGF-8b-induced proliferation. Inhibition of the FGF-8b-activated ERK/MAPK pathway decreased FGF-8b-stimulated proliferation. Blocking the constitutively active PI3K/Akt and p38 MAPK pathways also lowered FGF-8b-induced cyclin D1 expression and proliferation. Corresponding results were obtained in MCF-7 cells. In S115 and MCF-7 mouse tumours, FGF-8b increased cyclin D1 and Ki67 levels. Moreover, FGF-8b opposed staurosporine-induced S115 cell death which effect was blocked by inhibiting the PI3K/Akt pathway but not the ERK/MAPK pathway. In conclusion, our results suggest that FGF-8b increases breast cancer cell growth both by stimulating cell cycle progression and by protecting against cell death.
Emeli M Nilsson; Leon J S Brokken; Pirkko L H?rk?nen
Related Documents :
8946019 - Altered g1 checkpoint control determines adaptive survival responses to ionizing radiat...
16477379 - Sodium butyrate induces apoptosis and cell cycle arrest in primary effusion lymphoma ce...
8875049 - Cytometry of cyclin proteins.
20403319 - The hidden rhythms of the dividing cell.
18981479 - Differentiation of trophoblast stem cells into giant cells is triggered by p57/kip2 inh...
16517759 - The d-type cyclin cycd3;1 is limiting for the g1-to-s-phase transition in arabidopsis.
21472079 - Honey induces apoptosis in renal cell carcinoma.
19182209 - Caspase-8 dependent histone acetylation by a novel proteasome inhibitor, npi-0052: a me...
22954439 - From cell culture to a cure: pancreatic β-cell replacement strategies for diabetes mel...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-04
Journal Detail:
Title:  Experimental cell research     Volume:  316     ISSN:  1090-2422     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-22     Completed Date:  2010-03-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  800-12     Citation Subset:  IM    
Copyright Information:
2009 Elsevier Inc. All rights reserved.
Department of Laboratory Medicine, Tumour Biology, Lund University, CRC, Building 91, Plan 10, Entrance 72, UMAS, 20502 Malm?, Sweden.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
1-Phosphatidylinositol 3-Kinase / metabolism
Breast Neoplasms / metabolism*,  physiopathology
Cell Cycle / physiology*
Cell Death / physiology*
Cell Line, Tumor
Cell Proliferation
Cyclin D1 / genetics,  metabolism
Enzyme Activation
Enzyme Inhibitors / metabolism
Fibroblast Growth Factor 8 / genetics,  metabolism*
Mice, Nude
Protein Isoforms / genetics,  metabolism*
Proto-Oncogene Proteins c-akt / metabolism
RNA, Small Interfering / genetics,  metabolism
Signal Transduction / physiology
p38 Mitogen-Activated Protein Kinases / metabolism
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Protein Isoforms; 0/RNA, Small Interfering; 136601-57-5/Cyclin D1; 148997-75-5/Fibroblast Growth Factor 8; EC 3-Kinase; EC Proteins c-akt; EC Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Leukemia inhibitory factor dependent increase in myoblast cell number is associated with phosphotidy...
Next Document:  A conserved peptide motif in Raver2 mediates its interaction with the polypyrimidine tract-binding p...