| Fibroblast growth factor 8 increases breast cancer cell growth by promoting cell cycle progression and by protecting against cell death. | |
| | |
MedLine Citation:
|
PMID: 19962979 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Fibroblast growth factor 8 (FGF-8) is expressed in a large proportion of breast cancers, whereas its level in normal mammary gland epithelium is low. Previous studies have shown that FGF-8b stimulates breast cancer cell growth in vitro and in vivo. To explore the mechanisms by which FGF-8b promotes growth, we studied its effects on cell cycle regulatory proteins and signalling pathways in mouse S115 and human MCF-7 breast cancer cells. We also studied the effect of FGF-8b on cell survival. FGF-8b induced cell cycle progression and up-regulated particularly cyclin D1 mRNA and protein in S115 cells. Silencing cyclin D1 with siRNA inhibited most but not all FGF-8b-induced proliferation. Inhibition of the FGF-8b-activated ERK/MAPK pathway decreased FGF-8b-stimulated proliferation. Blocking the constitutively active PI3K/Akt and p38 MAPK pathways also lowered FGF-8b-induced cyclin D1 expression and proliferation. Corresponding results were obtained in MCF-7 cells. In S115 and MCF-7 mouse tumours, FGF-8b increased cyclin D1 and Ki67 levels. Moreover, FGF-8b opposed staurosporine-induced S115 cell death which effect was blocked by inhibiting the PI3K/Akt pathway but not the ERK/MAPK pathway. In conclusion, our results suggest that FGF-8b increases breast cancer cell growth both by stimulating cell cycle progression and by protecting against cell death. |
| | |
Authors:
|
Emeli M Nilsson; Leon J S Brokken; Pirkko L H?rk?nen |
Related Documents
:
|
8980229 - Dacapo, a cyclin-dependent kinase inhibitor, stops cell proliferation during drosophila... 8207059 - Cyclin-like accumulation and loss of the putative kinetochore motor cenp-e results from... 15796209 - Cell cycle and apoptosis deregulation in classical hodgkin lymphomas. 10194549 - Differential effects of ucn-01, staurosporine and cgp 41 251 on cell cycle progression ... 15922479 - Induction of anti-proliferative mechanisms in hepatitis b virus producing cells. 15817149 - Expression of progesterone receptor b is associated with g0/g1 arrest of the cell cycle... 10748199 - Regulation of membrane-type-1 matrix metalloproteinase activity by its cytoplasmic domain. 2960419 - T cell subsets in multiple sclerosis. gradients at plaque borders and differences in no... 19147009 - The primary cilium coordinates signaling pathways in cell cycle control and migration d... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-04 |
Journal Detail:
|
Title: Experimental cell research Volume: 316 ISSN: 1090-2422 ISO Abbreviation: Exp. Cell Res. Publication Date: 2010 Mar |
Date Detail:
|
Created Date: 2010-02-22 Completed Date: 2010-03-25 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: United States |
Other Details:
|
Languages: eng Pagination: 800-12 Citation Subset: IM |
Copyright Information:
|
2009 Elsevier Inc. All rights reserved. |
Affiliation:
|
Department of Laboratory Medicine, Tumour Biology, Lund University, CRC, Building 91, Plan 10, Entrance 72, UMAS, 20502 Malm?, Sweden. Emeli.Nilsson@med.lu.se |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
1-Phosphatidylinositol 3-Kinase
/
metabolism Animals Breast Neoplasms / metabolism*, physiopathology Cell Cycle / physiology* Cell Death / physiology* Cell Line, Tumor Cell Proliferation Cyclin D1 / genetics, metabolism Enzyme Activation Enzyme Inhibitors / metabolism Female Fibroblast Growth Factor 8 / genetics, metabolism* Humans Mice Mice, Nude Protein Isoforms / genetics, metabolism* Proto-Oncogene Proteins c-akt / metabolism RNA, Small Interfering / genetics, metabolism Signal Transduction / physiology p38 Mitogen-Activated Protein Kinases / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Enzyme Inhibitors; 0/Protein Isoforms; 0/RNA, Small Interfering; 136601-57-5/Cyclin D1; 148997-75-5/Fibroblast Growth Factor 8; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Leukemia inhibitory factor dependent increase in myoblast cell number is associated with phosphotidy...
Next Document: A conserved peptide motif in Raver2 mediates its interaction with the polypyrimidine tract-binding p...