Document Detail

Fibroblast growth factor-7 regulates stratification of the bladder urothelium.
MedLine Citation:
PMID:  11696824     Owner:  NLM     Status:  MEDLINE    
PURPOSE: The cellular and molecular mechanisms that regulate the organization of bladder urothelium into basal, intermediate and superficial cell layers remain poorly understood. We tested the hypothesis that fibroblast growth factor (FGF)-7 is essential for generating a multilayered stratified bladder epithelium. MATERIALS AND METHODS: The morphological and molecular characteristics of bladder urothelium in age and sex matched FGF-7 +/+ wild-type and -/- null mice were evaluated. In addition, the effect of exogenous FGF-7 on the growth and differentiation of primary murine urothelial cells was assessed. RESULTS: Morphometric analyses demonstrate that FGF-7 null urothelium is markedly thinned compared with wild-type urothelium. Electron microscopy revealed that null urothelium lacks the intermediate cell layers and molecular marker analyses confirmed this observation. In vitro cell culture experiments indicated that FGF-7 regulates urothelial cell growth, differentiation and stratification. Primary urothelial cultures maintained without FGF-7 ceased to divide and expressed proteins characteristic of terminally differentiated umbrella cells. In contrast, cultures maintained with exogenous FGF-7 contained proliferating epithelial cells with protein expression patterns consistent with those of intermediate cells in addition to terminally differentiated, post-mitotic umbrella cells. Importantly, isolated urothelial cells maintained with exogenous FGF-7 formed a multilayered epithelium in vitro. CONCLUSIONS: Collectively these data indicate that FGF-7 is essential for normal bladder urothelial stratification, specifically the formation of the intermediate cell layers. Fibroblast growth factor-7 stimulates urothelial proliferation and delays the differentiation of these cells into post-mitotic umbrella cells.
J A Tash; S G David; E D Vaughan ED; D A Herzlinger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of urology     Volume:  166     ISSN:  0022-5347     ISO Abbreviation:  J. Urol.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-11-06     Completed Date:  2001-12-31     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376374     Medline TA:  J Urol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2536-41     Citation Subset:  AIM; IM    
Department of Urology, James Buchanan Brady Urology Foundation, New York Presbyterian Hospital-Weill Medical College of Cornell University, New York, New York, USA.
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MeSH Terms
Cell Differentiation
Cells, Cultured
Fibroblast Growth Factor 7
Fibroblast Growth Factors / physiology*
Keratinocytes / physiology*
Mice, Inbred C57BL
Urinary Bladder / cytology*
Urothelium / cytology
Grant Support
Reg. No./Substance:
0/Fgf7 protein, mouse; 126469-10-1/Fibroblast Growth Factor 7; 62031-54-3/Fibroblast Growth Factors

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