| Fibroblast growth factor-2 stimulates directed migration of periodontal ligament cells via PI3K/AKT signaling and CD44/hyaluronan interaction. | |
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MedLine Citation:
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PMID: 20857427 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fibroblast growth factor-2 (FGF-2) regulates a variety of functions of the periodontal ligament (PDL) cell, which is a key player during tissue regeneration following periodontal tissue breakdown by periodontal disease. In this study, we investigated the effects of FGF-2 on the cell migration and related signaling pathways of MPDL22, a mouse PDL cell clone. FGF-2 activated the migration of MPDL22 cells and phosphorylation of phosphatidylinositol 3-kinase (PI3K) and akt. The P13K inhibitors, Wortmannin and LY294002, suppressed both cell migration and akt activation in MPDL22, suggesting that the PI3K/akt pathway is involved in FGF-2-stimulated migration of MPDL22 cells. Moreover, in response to FGF-2, MPDL22 showed increased CD44 expression, avidity to hyaluronan (HA) partly via CD44, HA production and mRNA expression of HA synthase (Has)-1, 2, and 3. However, the distribution of HA molecular mass produced by MPDL22 was not altered by FGF-2 stimulation. Treatment of transwell membrane with HA facilitated the migration of MPDL22 cells and an anti-CD44 neutralizing antibody inhibited it. Interestingly, the expression of CD44 was colocalized with HA on the migrating cells when stimulated with FGF-2. Furthermore, an anti-CD44 antibody and small interfering RNA for CD44 significantly decreased the FGF-2-induced migration of MPDL22 cells. Taken together, PI3K/akt and CD44/HA signaling pathways are responsible for FGF-2-mediated cell motility of PDL cells, suggesting that FGF-2 accelerates periodontal regeneration by regulating the cellular functions including migration, proliferation and modulation of extracellular matrix production. |
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Authors:
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Yoshio Shimabukuro; Hiroaki Terashima; Masahide Takedachi; Kenichiro Maeda; Tomomi Nakamura; Keigo Sawada; Mariko Kobashi; Toshihito Awata; Hiroyuki Oohara; Takanobu Kawahara; Tomoaki Iwayama; Tomoko Hashikawa; Manabu Yanagita; Satoru Yamada; Shinya Murakami |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular physiology Volume: 226 ISSN: 1097-4652 ISO Abbreviation: J. Cell. Physiol. Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2010-12-30 Completed Date: 2011-01-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 809-21 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Wiley-Liss, Inc. |
Affiliation:
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Division of Oral Biology and Disease Control, Department of Periodontology, Osaka University Graduate School of Dentistry, Osaka, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD44 / metabolism* Cell Movement / drug effects* Fibroblast Growth Factor 2 / pharmacology* Gene Silencing / drug effects Humans Hyaluronic Acid / metabolism* Mice Mice, Inbred BALB C Periodontal Ligament / cytology*, drug effects, enzymology Phosphatidylinositol 3-Kinase / metabolism* Phosphorylation / drug effects Protein Binding / drug effects Proto-Oncogene Proteins c-akt / metabolism* Pseudopodia / drug effects, metabolism RNA, Small Interfering / metabolism Signal Transduction / drug effects Up-Regulation / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD44; 0/RNA, Small Interfering; 103107-01-3/Fibroblast Growth Factor 2; 9004-61-9/Hyaluronic Acid; EC 2.7.1.137/Phosphatidylinositol 3-Kinase; EC 2.7.11.1/Proto-Oncogene Proteins c-akt |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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