Document Detail

Fibroblast growth factor-2 regulates the synthesis of hyaluronan by human periodontal ligament cells.
MedLine Citation:
PMID:  15573376     Owner:  NLM     Status:  MEDLINE    
Basic fibroblast growth factor (FGF-2) can enhance biological potentials of periodontal ligament cells and its topical application induces considerable periodontal tissue regeneration in vivo. In this study, we examined the effect of FGF-2 on the production of hyaluronan (HA), an extracellular matrix playing important roles in homeostasis and inflammatory/wound healing responses, by human periodontal ligament (HPDL) cells. An inhibition binding-protein assay revealed that FGF-2 significantly increased HA production by HPDL cells in a dose dependent manner. Analysis by HPLC revealed that in conditioned medium of FGF-2-treated HPDL cells HA had a higher molecular mass, compared to that of untreated HPDL cells. RT-PCR analysis revealed the enhancement of mRNA expression of hyaluronan synthase (HAS) 1 and HAS 2, both of which contribute to the production of HA with a high molecular mass, but not HAS 3 in the FGF-2-treated HPDL cells. In contrast, three isoforms of hyaluronidase (HYAL) transcript were unchanged in the FGF-2-treated HPDL cells. These results provide new evidence for the possible involvement of FGF-2 in the regulation of HA production and its appreciable roles in not only homeostasis but also regeneration of periodontal tissues.
Yoshio Shimabukuro; Tomoo Ichikawa; Shinichi Takayama; Satoru Yamada; Masahide Takedachi; Mami Terakura; Tomoko Hashikawa; Shinya Murakami
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  203     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-03-31     Completed Date:  2005-07-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  557-63     Citation Subset:  IM    
Copyright Information:
Copyright 2004 Wiley-Liss, Inc.
Department of Periodontology, Division of Oral Biology and Disease Control, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan.
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MeSH Terms
Cells, Cultured
Culture Media, Conditioned / pharmacology
Dose-Response Relationship, Drug
Extracellular Matrix / drug effects,  metabolism*
Fibroblast Growth Factor 2 / pharmacology,  physiology*
Glucuronosyltransferase / genetics
Homeostasis / drug effects,  physiology
Hyaluronic Acid / biosynthesis*
Hyaluronoglucosaminidase / drug effects,  metabolism
Periodontal Ligament / drug effects,  metabolism*
Protein Isoforms / drug effects,  metabolism
RNA, Messenger / drug effects,  metabolism
Regeneration / drug effects,  physiology
Up-Regulation / drug effects,  physiology
Wound Healing / drug effects,  physiology
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Protein Isoforms; 0/RNA, Messenger; 103107-01-3/Fibroblast Growth Factor 2; 9004-61-9/Hyaluronic Acid; EC 2.4.1.-/HAS2 protein, human; EC; EC synthase; EC

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