Document Detail

Fibroblast growth factor 2 (FGF-2) promotes acquisition of epidermal growth factor (EGF) responsiveness in mouse striatal precursor cells: identification of neural precursors responding to both EGF and FGF-2.
MedLine Citation:
PMID:  9742155     Owner:  NLM     Status:  MEDLINE    
Epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF-2) induce the proliferation of neural precursor cells isolated from specific regions of the embryonic and adult brain. However, the lineage relationship between the EGF- and FGF-2-responsive cells is unknown. In this study we used phosphorylation of the transcription factor cAMP response element-binding protein as a functional readout to identify cells responding to EGF and FGF-2. In primary cultures of mouse embryonic day 14 (E14) striatum, maintained in vitro for 24 hr, 12% of the cells responded to FGF-2, whereas no response to EGF could be detected. Seventy-five percent of these FGF-2-responsive cells were beta tubulin III (TuJ1)-positive neurons, and 25% expressed nestin, a marker for neuroepithelial precursors. After growth factor treatment for 6 d, a population of nestin-positive cells responding to both EGF and FGF-2 were identified. The 6-d-old cultures also contained a small number of TuJ1-positive cells that responded to FGF-2 only. Priming of striatal cells for 24 hr with FGF-2 but not with EGF was sufficient to induce the appearance of EGF- and FGF-2 responsive cells after only 2 d in vitro. Thus, neural precursor cells from the mouse E14 striatum initially responding to FGF-2 only acquire EGF responsiveness later during in vitro development. At this stage EGF and FGF-2 act on the same cells. The acquisition of EGF responsiveness is promoted by FGF-2.
F Ciccolini; C N Svendsen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  18     ISSN:  0270-6474     ISO Abbreviation:  J. Neurosci.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1998-10-09     Completed Date:  1998-10-09     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7869-80     Citation Subset:  IM    
Medical Research Council Cambridge Centre for Brain Repair, Cambridge CB2 2PY, England.
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MeSH Terms
Antibodies, Monoclonal
Cell Differentiation / drug effects
Cells, Cultured
Corpus Striatum / cytology*
Cyclic AMP Response Element-Binding Protein / analysis,  metabolism
Epidermal Growth Factor / analysis,  pharmacology*
Fetus / cytology
Fibroblast Growth Factor 2 / pharmacology*
Intermediate Filament Proteins / analysis
Mice, Inbred Strains
Nerve Tissue Proteins*
Neurons / chemistry,  cytology*,  drug effects
Stem Cells / chemistry,  cytology*,  drug effects
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Cyclic AMP Response Element-Binding Protein; 0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/nestin; 103107-01-3/Fibroblast Growth Factor 2; 62229-50-9/Epidermal Growth Factor

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