Document Detail


Fibroblast cytoskeletal remodeling induced by tissue stretch involves ATP signaling.
MedLine Citation:
PMID:  23460361     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fibroblasts in whole areolar connective tissue respond to static stretching of the tissue by expanding and remodeling their cytoskeleton within minutes both ex vivo and in vivo. This study tested the hypothesis that the mechanism of fibroblast expansion in response to tissue stretch involves extracellular ATP signaling. In response to tissue stretch ex vivo, ATP levels in the bath solution increased significantly, and this increase was sustained for 20 min, returning to baseline at 60 min. No increase in ATP was observed in tissue incubated without stretch or tissue stretched in the presence of the Rho kinase inhibitor Y27632. The increase in fibroblast cross sectional area in response to tissue stretch was blocked by both suramin (a purinergic receptor blocker) and apyrase (an enzyme that selectively degrades extracellular ATP). Furthermore, connexin channel blockers (octanol and carbenoxolone), but not VRAC (fluoxetine) or pannexin (probenecid) channel blockers, inhibited fibroblast expansion. Together, these results support a mechanism in which extracellular ATP signaling via connexin hemichannels mediate the active change in fibroblast shape that occurs in response to a static increase in tissue length.
Authors:
Helene M Langevin; Takumi Fujita; Nicole A Bouffard; Takahiro Takano; Cathryn Koptiuch; Gary J Badger; Maiken Nedergaard
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  228     ISSN:  1097-4652     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2013 Sep 
Date Detail:
Created Date:  2013-05-28     Completed Date:  2013-09-03     Revised Date:  2014-09-02    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1922-6     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / genetics,  metabolism*
Amides / pharmacology
Animals
Carbenoxolone / pharmacology
Cell Communication / drug effects
Cells, Cultured
Connective Tissue / drug effects*,  metabolism
Cytoskeleton / drug effects,  genetics,  metabolism*
Fibroblasts / cytology,  drug effects
Mice
Pyridines / pharmacology
Signal Transduction / drug effects,  genetics*
Stress, Mechanical
Suramin / pharmacology
rho-Associated Kinases / antagonists & inhibitors,  genetics*
Grant Support
ID/Acronym/Agency:
R01 AT001121/AT/NCCAM NIH HHS; R01 DE022743/DE/NIDCR NIH HHS; R01 NS075177/NS/NINDS NIH HHS; R01 NS078167/NS/NINDS NIH HHS; R01 NS078304/NS/NINDS NIH HHS; R01-AT01121/AT/NCCAM NIH HHS
Chemical
Reg. No./Substance:
0/Amides; 0/Pyridines; 138381-45-0/Y 27632; 6032D45BEM/Suramin; 8L70Q75FXE/Adenosine Triphosphate; EC 2.7.11.1/rho-Associated Kinases; MM6384NG73/Carbenoxolone
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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