| Fibrinolysis-inhibitory capacity of clot-embedded surfactant is enhanced by SP-B and SP-C. | |
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MedLine Citation:
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PMID: 12388357 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Incorporation of pulmonary surfactant into fibrin inhibits its plasmic degradation. In the present study we investigated the influence of surfactant proteins (SP)-A, SP-B, and SP-C on the fibrinolysis-inhibitory capacity of surfactant phospholipids. Plasmin-induced fibrinolysis was quantified by means of a (125)I-fibrin plate assay, and surfactant incorporation into polymerizing fibrin was analyzed by measuring the incorporation of (3)H-labeled L-alpha-dipalmitoylphosphatidylcholine into the insoluble clot material. Incorporation of a calf lung surfactant extract (Alveofact) and an organic extract of natural rabbit large surfactant aggregates (LSA) into a fibrin clot revealed a stronger inhibitory effect on plasmic cleavage of this clot than a synthetic phospholipid mixture (PLX) and unprocessed LSA. Reconstitution of PLX with SP-B and SP-C increased, whereas reconstitution with SP-A decreased, the fibrinolysis-inhibitory capacity of the phospholipids. The SP-B effect was paralleled by an increased incorporation of phospholipids into fibrin. We conclude that the inhibitory effect of surfactant incorporation into polymerizing fibrin on its susceptibility to plasmic cleavage is enhanced by SP-B and SP-C but reduced by SP-A. In the case of SP-B, increased phospholipid incorporation may underlie this finding. |
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Authors:
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Philipp Markart; Clemens Ruppert; Friedrich Grimminger; Werner Seeger; Andreas Günther |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2002-09-06 |
Journal Detail:
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Title: American journal of physiology. Lung cellular and molecular physiology Volume: 284 ISSN: 1040-0605 ISO Abbreviation: Am. J. Physiol. Lung Cell Mol. Physiol. Publication Date: 2003 Jan |
Date Detail:
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Created Date: 2002-12-09 Completed Date: 2003-01-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100901229 Medline TA: Am J Physiol Lung Cell Mol Physiol Country: United States |
Other Details:
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Languages: eng Pagination: L69-76 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine, Justus-Liebig-University-Giessen, Klinikstrasse 36, D-35385 Giessen, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Coagulation / physiology* Cattle Drug Combinations Fibrin / metabolism Fibrinolysis / drug effects, physiology* Lipids / pharmacology Phospholipids / metabolism, pharmacology Pulmonary Surfactant-Associated Protein B / pharmacology, physiology* Pulmonary Surfactant-Associated Protein C / pharmacology, physiology* Pulmonary Surfactants / metabolism*, pharmacology Rabbits |
| Chemical | |
Reg. No./Substance:
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0/Drug Combinations; 0/Lipids; 0/Phospholipids; 0/Pulmonary Surfactant-Associated Protein B; 0/Pulmonary Surfactant-Associated Protein C; 0/Pulmonary Surfactants; 0/SF-RI 1, bovine surfactant preparation; 9001-31-4/Fibrin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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