Document Detail


Fibrin microbeads (FMB) as a 3D platform for kidney gene and cell therapy.
MedLine Citation:
PMID:  16395256     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell and gene therapy may alter the outcome of renal diseases, such as hereditary nephropathies, acute and chronic glomerulonephritis and allograft nephropathy. However, owing to blockade of many viral and cellular vehicles by the complex glomerular architecture, the exact nature of gene and cell delivery into specific renal compartments remains currently unknown. To study the interaction of viral vectors with a variety of renal cells and mesenchymal stem cells (MSCs), we employed a novel biological three-dimensional (3D) matrix comprised of fibrin microbeads (FMB) in comparison to monolayer cell culture. Our studies showed that renal cells of both established and primary lines can grow efficiently on FMB and differentiate into epithelial structures, as shown by electron microscopy. Gene delivery into renal cells in 3D was observed for several viral vectors and growth in 3D on FMB conferred resistance to renal cancer cells in the context of oncolytic adenoviruses. Finally, MSCs from various rodent species attached to FMB, grew robustly, survived for several weeks and could efficiently be transduced on FMB. Thus, on the basis of growth, differentiation and transduction of renal cells in 3D, FMB emerge as a novel 3D cellular microenvironment that differs substantially from monolayer cell cultures.
Authors:
N Shimony; R Gorodetsky; G Marx; D Gal; R Rivkin; A Ben-Ari; A Landsman; Y S Haviv
Related Documents :
667136 - Collagen synthesis by human glomerular cells in culture.
8731086 - Expression of a nonmuscle myosin heavy chain in glomerular cells differentiates various...
6405396 - Inhibition of prostaglandin synthetases derived from neuronal and glial cells and rat r...
658136 - The myorelaxant effect of theophylline on smooth muscle cells of the afferent arteriole...
8643386 - The central region of the yeast u4 snrna is not important for hammerhead catalysis, but...
12067376 - Escherichia coli survival in groundwater and effluent measured using a combination of p...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Kidney international     Volume:  69     ISSN:  0085-2538     ISO Abbreviation:  Kidney Int.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-26     Completed Date:  2006-07-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0323470     Medline TA:  Kidney Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  625-33     Citation Subset:  IM    
Affiliation:
Cell and Gene Therapy Program, Department of Medicine, Division of Nephrology, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Differentiation
Cell Line
Cell Proliferation
Cell Survival
Fibrin*
Gene Therapy / instrumentation,  methods*
Humans
Kidney / cytology,  pathology,  ultrastructure
Kidney Diseases / pathology,  therapy*
Kidney Neoplasms / pathology,  therapy,  ultrastructure,  virology
Mesenchymal Stem Cells / cytology,  ultrastructure
Microscopy, Electron
Microspheres*
Oncolytic Viruses / physiology
Chemical
Reg. No./Substance:
9001-31-4/Fibrin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Insulin resistance and impaired myocardial fatty acid metabolism in dialysis patients with normal co...
Next Document:  Angiotensin-converting enzyme (ACE) inhibition in type 2, diabetic patients-- interaction with ACE i...