Document Detail

Fibrin microbeads (FMB) as a 3D platform for kidney gene and cell therapy.
MedLine Citation:
PMID:  16395256     Owner:  NLM     Status:  MEDLINE    
Cell and gene therapy may alter the outcome of renal diseases, such as hereditary nephropathies, acute and chronic glomerulonephritis and allograft nephropathy. However, owing to blockade of many viral and cellular vehicles by the complex glomerular architecture, the exact nature of gene and cell delivery into specific renal compartments remains currently unknown. To study the interaction of viral vectors with a variety of renal cells and mesenchymal stem cells (MSCs), we employed a novel biological three-dimensional (3D) matrix comprised of fibrin microbeads (FMB) in comparison to monolayer cell culture. Our studies showed that renal cells of both established and primary lines can grow efficiently on FMB and differentiate into epithelial structures, as shown by electron microscopy. Gene delivery into renal cells in 3D was observed for several viral vectors and growth in 3D on FMB conferred resistance to renal cancer cells in the context of oncolytic adenoviruses. Finally, MSCs from various rodent species attached to FMB, grew robustly, survived for several weeks and could efficiently be transduced on FMB. Thus, on the basis of growth, differentiation and transduction of renal cells in 3D, FMB emerge as a novel 3D cellular microenvironment that differs substantially from monolayer cell cultures.
N Shimony; R Gorodetsky; G Marx; D Gal; R Rivkin; A Ben-Ari; A Landsman; Y S Haviv
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Kidney international     Volume:  69     ISSN:  0085-2538     ISO Abbreviation:  Kidney Int.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-26     Completed Date:  2006-07-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0323470     Medline TA:  Kidney Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  625-33     Citation Subset:  IM    
Cell and Gene Therapy Program, Department of Medicine, Division of Nephrology, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
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MeSH Terms
Cell Differentiation
Cell Line
Cell Proliferation
Cell Survival
Gene Therapy / instrumentation,  methods*
Kidney / cytology,  pathology,  ultrastructure
Kidney Diseases / pathology,  therapy*
Kidney Neoplasms / pathology,  therapy,  ultrastructure,  virology
Mesenchymal Stem Cells / cytology,  ultrastructure
Microscopy, Electron
Oncolytic Viruses / physiology
Reg. No./Substance:

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