Document Detail


Fibrin architecture in clots: a quantitative polarized light microscopy analysis.
MedLine Citation:
PMID:  19054699     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fibrin plays a vital structural role in thrombus integrity. Thus, the ability to assess fibrin architecture has a potential to provide insight into thrombosis and thrombolysis. Fibrin has an anisotropic molecular structure, which enables it to be seen with polarized light. Therefore, we aimed to determine if automated polarized light microscopy methods of quantifying two structural parameters; fibrin fiber bundle orientation and fibrin's optical retardation (OR: a measure of molecular anisotropy) could be used to assess thrombi. To compare fibrin fiber bundle orientation we analyzed picrosirius red-stained sections obtained from clots formed: (A) in vitro, (B) in injured and stenotic coronary arteries, and (C) in surgically created aortic aneurysms (n=6 for each group). To assess potential changes in OR, we examined fibrin in picrosirius red-stained clots formed after ischemic preconditioning (10 min ischemia+10 min reflow; a circumstance shown to enhance lysability) and in control clots (n=8 each group). The degree of fibrin organization differed significantly according to the location of clot formation; fibrin was most aligned in the aneurysms and least aligned in vitro whereas fibrin in the coronary clots had an intermediate organization. The OR of fibrin in the clots formed after ischemic preconditioning was lower than that in controls (2.9+/-0.5 nm versus 5.4+/-1.0 nm, P<0.05). The automated polarized light analysis methods not only enabled fibrin architecture to be assessed, but also revealed structural differences in clots formed under different circumstances.
Authors:
Peter Whittaker; Karin Przyklenk
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural     Date:  2008-12-02
Journal Detail:
Title:  Blood cells, molecules & diseases     Volume:  42     ISSN:  1096-0961     ISO Abbreviation:  Blood Cells Mol. Dis.     Publication Date:    2009 Jan-Feb
Date Detail:
Created Date:  2008-12-29     Completed Date:  2009-02-09     Revised Date:  2010-09-22    
Medline Journal Info:
Nlm Unique ID:  9509932     Medline TA:  Blood Cells Mol Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  51-6     Citation Subset:  IM    
Affiliation:
Cardiovascular Research Institute, Department of Emergency Medicine, Wayne State University, School of Medicine, Detroit, MI 48201, USA. peter.whittaker@wayne.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Coagulation / physiology
Coloring Agents
Fibrin / ultrastructure*
Humans
Microscopy, Polarization / methods*
Thrombosis / blood*
Grant Support
ID/Acronym/Agency:
R01 HL 72684/HL/NHLBI NIH HHS; R01 HL072684-03/HL/NHLBI NIH HHS; R56 HL072684-05/HL/NHLBI NIH HHS; R56 HL72684/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Coloring Agents; 9001-31-4/Fibrin
Comments/Corrections

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