Document Detail


Fibrillarin, a nucleolar protein, is required for normal nuclear morphology and cellular growth in HeLa cells.
MedLine Citation:
PMID:  17603021     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fibrillarin is a key small nucleolar protein in eukaryotes, which has an important role in pre-rRNA processing during ribosomal biogenesis. Though several functions of fibrillarin are known, its function during the cell cycle is still unknown. In this study, we confirmed the dynamic localization of fibrillarin during the cell cycle of HeLa cells and also performed functional studies by using a combination of immunofluorescence microscopy and RNAi technique. We observed that depletion of fibrillarin has almost no effect on the nucleolar structure. However, fibrillarin-depleted cells showed abnormal nuclear morphology. Moreover, fibrillarin depletion resulted in the reduction of the cellular growth and modest accumulation of cells with 4n DNA content. Our data suggest that fibrillarin would play a critical role in the maintenance of nuclear shape and cellular growth.
Authors:
Mohammed Abdullahel Amin; Sachihiro Matsunaga; Nan Ma; Hideaki Takata; Masami Yokoyama; Susumu Uchiyama; Kiichi Fukui
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-25
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  360     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-13     Completed Date:  2007-08-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  320-6     Citation Subset:  IM    
Affiliation:
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Osaka, Japan.
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle / physiology*
Cell Cycle Proteins / metabolism
Cell Enlargement*
Cell Nucleus / metabolism*,  ultrastructure*
Cell Proliferation*
Chromosomal Proteins, Non-Histone / metabolism*
Hela Cells
Humans
Nuclear Proteins / metabolism*
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Chromosomal Proteins, Non-Histone; 0/Nuclear Proteins; 0/fibrillarin

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