Document Detail


Fibre type-specific change in FXYD1 phosphorylation during acute intense exercise in humans.
MedLine Citation:
PMID:  23359667     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of the present study was to examine fibre type-specific Na(+)-K(+) pump subunit expression and exercise-induced alterations in phospholemman (FXYD1) phosphorylation in humans. Segments of human skeletal muscle fibres were dissected and fibre typed, and protein expression was determined by Western blotting. The protein expression of the Na(+)-K(+) pump α2 isoform was lower in type I than in type II fibres (0.63 ± 0.04 a.u. vs. 1.00 ± 0.07 a.u., P < 0.001), while protein expression of the Na(+)-K(+) pump α1 and β1 isoforms was not different. Protein expression of the ATP-dependent potassium channel Kir6.2 was higher in type I compared with type II fibres. In both type I (P < 0.01) and type II fibres (P < 0.001) the AB_FXYD1 signal was lower after exercise compared with rest, indicating an increase in unspecific FXYD1 phosphorylation. The FXYD1 serine 68 phosphorylation was higher (P < 0.001) after exercise compared with rest in type II fibres (1.90 ± 0.25 vs. 1.00 ± 0.08) and not changed in type I fibres. Total FXYD1 was not expressed in a fibre type-specific manner. Expression of phosphofructokinase was lower (P < 0.001) in type I than in type II fibres, whereas citrate synthase and 3-hydroxyacyl-CoA dehydrogenase were more abundant (P < 0.001) in type I fibres. In conclusion, FXYD1 phosphorylation at serine 68 increased after an acute bout of intense exercise in human type II fibres, while AB_FXYD1 signal intensity was lower in both type I and type II fibres, indicating fibre type-specific differences in FXYD1 phosphorylation on serine 63, serine 68 and threonine 69. This, together with the observation of a higher abundance of the Na(+)-K(+) pump α2 isoform protein in type II fibres, is likely to have importance for the exercise-induced human Na(+)-K(+) pump activity in the different fibre types.
Authors:
Martin Thomassen; Robyn M Murphy; Jens Bangsbo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-28
Journal Detail:
Title:  The Journal of physiology     Volume:  591     ISSN:  1469-7793     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-18     Completed Date:  2013-08-30     Revised Date:  2014-03-26    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1523-33     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Exercise*
Gene Expression
Humans
Male
Membrane Proteins / metabolism*
Muscle Fibers, Fast-Twitch / metabolism*
Muscle Fibers, Slow-Twitch / metabolism*
Organ Specificity
Phosphofructokinases / genetics,  metabolism
Phosphoproteins / metabolism*
Phosphorylation
Potassium Channels, Inwardly Rectifying / genetics,  metabolism
Protein Isoforms / genetics,  metabolism
Sodium-Potassium-Exchanging ATPase / chemistry,  genetics,  metabolism
Chemical
Reg. No./Substance:
0/Kir6.2 channel; 0/Membrane Proteins; 0/Phosphoproteins; 0/Potassium Channels, Inwardly Rectifying; 0/Protein Isoforms; 135541-82-1/phospholemman; EC 2.7.1 -/Phosphofructokinases; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase
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