Document Detail


Fibered confocal fluorescence microscopy (Cell-viZio) facilitates extended imaging in the field of microcirculation. A comparison with intravital microscopy.
MedLine Citation:
PMID:  15467299     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study investigated the capability of fibered confocal fluorescence microscopy (FCFM) to provide in vivo microvascular observations. FCFM is specifically designed for in vivo in situ observation thanks to a probe composed of a fiber bundle and micro-optics having a diameter as small as 650 microm. In the first part of the study, we compared the main characteristics of FCFM with those of intravital fluorescence microscopy (IFM). A mouse cremaster preparation was used as a common basis to allow for imaging with both modalities. We discussed the feasibility of obtaining quantitative measurements usually provided by IFM in the context of FCFM: morphometry, capillary permeability, functional capillary density, vasoconstriction and dilation effects. In addition, the possibility to visualize fluorescent red blood cells or leukocytes was also evaluated. Phototoxicity issues and limitations of FCFM were also discussed. We showed that FCFM allows observations and measurements usually provided by IFM and that the real-time capability of the system, as well as the flexibility and small diameter of the optical probe enable micro-invasiveness and can extend imaging capabilities for in vivo in situ observations when compared to IFM.
Authors:
Elisabeth Laemmel; Magalie Genet; Georges Le Goualher; Aymeric Perchant; Jean-François Le Gargasson; Eric Vicaut
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2004-09-30
Journal Detail:
Title:  Journal of vascular research     Volume:  41     ISSN:  1018-1172     ISO Abbreviation:  J. Vasc. Res.     Publication Date:    2004 Sep-Oct
Date Detail:
Created Date:  2004-11-23     Completed Date:  2004-12-21     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9206092     Medline TA:  J Vasc Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  400-11     Citation Subset:  IM    
Affiliation:
Laboratoire d'Etude de la Microcirculation, Université Paris 7, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Capillaries / physiology
Capillary Permeability / physiology
Conjunctiva / blood supply
Dermatitis, Phototoxic
Erythrocytes / physiology
Fiber Optic Technology / instrumentation*,  methods*,  standards
Kidney / blood supply
Liver / blood supply
Male
Mice
Mice, Inbred BALB C
Microscopy, Confocal / instrumentation*,  methods*,  standards
Muscle, Skeletal / blood supply*
Platelet Aggregation / physiology
Reproducibility of Results
Splanchnic Circulation / physiology
Surgical Procedures, Minimally Invasive

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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