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Fia-spectrophotometric determination of thiamine after UV-irradiation.
MedLine Citation:
PMID:  18966184     Owner:  NLM     Status:  In-Data-Review    
The determination of thiamine was carried out by UV-photodegradation in a single-line flow-injection assembly. The UV-photodegradation of thiamine was carried out in the coil of the injection valve, constituted of a PTFE tubing, half of its length being helically coiled around a UV lamp. A peak with two adjoining maxima was produced by injection, corresponding to the absorbance of the irradiated and non-irradiated sample. The analytical parameter is the difference between the two peaks, measured at 264 nm. The calibration graph is linear over the 1.2-30 mug/ml range of thiamine hydrochloride in 0.1M HCl. The influence of certain admixed substances was studied and the method was tested for the determination of thiamine in tablets.
A F Daneţ; J M Calatayud
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Talanta     Volume:  41     ISSN:  0039-9140     ISO Abbreviation:  Talanta     Publication Date:  1994 Dec 
Date Detail:
Created Date:  2008-10-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  2147-51     Citation Subset:  -    
Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, Bul. Carol I, 13, Bucharest 70031, Romania.
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